Objective To explore the effects of berberine(BBR)on the proliferation,migration and apoptosis of MDA-MB-231 cells in triple-negative breast cancer(TNBC)and its possible mechanisms.Methods The 0,25,and 50 μmol/L of BBR were added as the experimental concentrations in each group to observe the effects of BBR on the growing morphology of MDA-MB-231 cells.A colony formation experiment was conducted to assess the dependence of BBR on cell proliferation ability.The migrational ability of cells was evaluated by Scratch and Transwell migration experiments.Annexin V/PI staining was used to detect the effect of BBR on cell apoptosis rate.Western blot was performed to examine the changes in protein expression levels of P65,phosphorylated P65,and apoptosis-related protein Caspase-3,after the action of BBR.Results The growing morphology of MDA-MB-231 cells was significantly changed with the addition of BBR under the light microscope.The colony formation assay demonstrated that BBR inhibited the proliferation of MDA-MB-231 cells in a concentration-dependent manner(P＜0.05).The Transwell results and the Scratch assay both demonstrated that BBR inhibited cell migration(P＜0.05).Annexin V/PI staining showed an increase of the apoptosis rate with the increase of BBR concentration(P＜0.05).Western blot assay showed no significant difference in P65 expression with the effect of BBR(P＞0.05),and the expression level of phosphorylated P65 protein was significantly inhibited,and the cleavage of apoptosis-related protein Caspase-3 increased(P＜0.05).Conclusion BBR can inhibit the proliferation and migration of MDA-MB-231 cells and induce the occurrence of apoptosis,and its mechanism may be related to the NF-κB signaling pathway.
berberinetriple-negative breast cancerNF-κBproliferationmigrationapoptosis
维普
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