Objective To investigate the effects of miR-210 via nuclear factor kappa-B(NF-κB)signaling pathway on the proliferation,apoptosis,invasion and metastasis of gastric cancer cells.Method qRT-PCR test was used to detect and compare expression of miR-210 in normal gastric mucosal epithelial cells and MGC803 gastric cancer cells.According to processing differences,gastric cancer cells were divided into blank control group,negative control group(miR-210 inhibitor control)and experimental group(miR-210 inhibitor).The proliferation,apoptosis,invasion and metastasis after transfection were compared by qRT-PCR tested miR-210 levels in all groups;and protein levels of IκBα,p-IκBα,NF-κB,and p-NF-KB in each group were compared.Results Compared with normal gastric mucosal epithelial cells,the levels of miR-210 in MGC803 gastric cancer cells increased(P＜0.05).Compared with the blank control group and negative control group,the experimental group showed a decrease in miR-210 expression,cell proliferation ability,invasion and metastasis ability,but an increase in cell apoptosis rate(P＜0.05),IκBα,p-IKBα,NF-κB,and p-NF-KB protein levels all decreased,differences were statistically significant(P＜0.05).Conclusion Expression of miR-210 increases significantly in MGC803 gastric cancer cells.miR-210 can inhibit the proliferation,invasion,and metastasis of gastric cancer cells and promote gastric cancer cell apoptosis by inhibiting the NF-κB signaling pathway.
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