Objective To investigate the single nucleotide polymorphisms(SNP)at rs7216389 and rs12603332 of serum orosomueo id 1-like protein 3(ORMDL3)gene and the relationship between this gene-environment interaction and childhood asthma.Methods A total of 102 children with asthma were chosen as asthma group,and 54 healthy children as control group.Alleles and genotype frequency differences of ORMDL3 gene rs7216389 and rs12603332 were compared between the two groups;logistic regression was used to analyze the relationship between ORMDL3 gene and the risk of childhood asthma;Haploview software was used to perform linkage disequilibrium and haplotype analysis.Generalized multifactor dimensionality reduction(GMDR)analyzed the interactions between ORMDL3 genes and the environment.Results The TT and TC genotype frequencies of ORMDL3 gene rs7216389 asthma group were higher than those of control group(P＜0.05),there was no difference in allele frequency distribution between the two groups(P＞0.05),there was no difference in allele and genotype frequency distribution between the two groups at rs12603332(P＞0.05).The risk of asthma in TT and TC genotype of rs7216389 was 7.000 times and 8.312 times greater than that of in CC genotype children,respectively(P＜0.05).The risk of asthma in rs12603332 AG genotype children was 4.266 times greater than that of AA genotype children(P＜0.05).The two loci showed strong linkage disequilibrium(D=1),and the distribution of haplotype TG,CA and TA were not statistically different between the two groups(P＞0.05).GMDR showed interaction between the two loci(P＜0.05),rs7216389 locus and eczema history,family history of allergy,atopic interaction(P＜0.05).Conclusion rs7216389 and rs12603332 polymorphisms of ORMDL3 gene are associated with the risk of asthma in children,the interaction between the two loci and rs7216389 loci is associated with eczema history,family history of allergy,and atopic activity.
关键词
血清类黏蛋白1样蛋白3/哮喘/单核苷酸多态性/交互作用/儿童
Key words
orosomueo id 1-like protein 3/asthma/single nucleotide polymorphism/interaction/chil-dren
维普
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