Objective To investigate the intracellular mechanism of Musca domestica antifungal peptide-1A(MAF-1A)mutant-22S3(Mt-22S3)in Candida albicans(C.albicans).Methods C.albicans cells in logarithmic growth were treated with 0,125,250,and 500 mg/L of Mt-22S3 for 12 hours.Fluorescence microscopy and fluorometric assays assessed changes in intracellular reactive oxygen species(ROS)and mitochondrial membrane potential following treatment.The effects on cell apoptosis were determined using the Annexin V-FITC/PI double staining method,while DNA interactions were analyzed via agarose gel electrophoresis.Results Mt-22S3 treatment resulted in a concentration-dependent increase in intracellular ROS(P＜0.05)and a decrease in mitochondrial membrane potential(P＜0.01),indicating mitochondrial depolarization.Enhanced apoptosis and necrosis were observed at higher concentrations of Mt-22S3.The gel retardation assay revealed significant DNA binding activity by Mt-22S3,suggesting a mode of antimicrobial action.Conclusion Mt-22S3 enters C.albicans cells,inducing ROS accumulation,mitochondrial depolarization,apoptosis,and DNA binding,demonstrating its potential as an effective antimicrobial strategy.
Candida albicansreactive oxygen speciesapoptosisantimicrobial peptidesmitochondrial membrane potentialDNA gel retardation
维普
万方数据
