Establishment of high performance liquid chromatography fingerprint and determination of polysaccharide content in Polygonatum kingianum from different origins
Establishment of high performance liquid chromatography fingerprint and determination of polysaccharide content in Polygonatum kingianum from different origins
Objective To investigate establishment of high performance liquid chromatography(HPLC)fingerprints and determination of polysaccharides content in Polygonatum kingianum from different origins.Methods Nine batches of Polygonatum kingianum(1 g)from Guizhou(Jianhe,Anshun,Tianzhu,Tongren,Chishui,Guiyang,and Nayong),Baise Guangxi,and Neijiang Sichuan sources were taken to prepare the test solution(1 g from each origin).The separation was performed on a DiKMA Platisll ODS C18(250 mm × 4.6 mum,5 μm)column(with acetonitrile-0.05％phosphoric acid aqueous solution as mobile phase in gradient elution at a flow rate of 1 mL/min and a column temperature of 35 ℃ with an injection volume of 20 μL and a detection wavelength of 203 nm)to establish HPLC fingerprints of Polygonatum kingianum extracts;the quality of Polygonatum kingianum from different origins was evaluated by similarity evaluation,principal component analysis and cluster analysis;the content of polysaccharides of Polygonatum kingianum from each batch was determined by anthrone-sulfuric acid method.Results HPLC fingerprints were established for nine batches of Polygonatum kingianum,and a total of 12 common peaks were identified.The polysaccharide content in all nine batches from different sources exceeded 7％.Among them,the descending order of polysaccharide content was as follows:Jianhe Guizhou＞Anshun Guizhou＞Tianzhu Guizhou＞Tongren Guizhou＞Chishui Guizhou＞Nayong Guizhou＞Baise Guangxi＞Neijiang Sichuan.The similarity analysis results indicated that the similarities between Neijiang Sichuan,Guiyang Guizhou and Baise Guangxi were 0.762,0.809,and 0.846,respectively;while the similarity of Polygonatum kingianum between other areas in Guizhou province were ≥ 0.853.Furthermore,three principal component factors were obtained using principal component analysis.Cluster analysis revealed that Polygonatum kingianum could be categorized into three distinct groups and content of polysaccharides of Polygonatum kingianum were ≥7.16％.Conclusion The method of HPLC fingerprints and determination of polysaccharides content in Polygonatum kingianum is simple,feasible,accurate,and reliable.
维普
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