不同产地滇黄精高效液相色谱法指纹图谱的建立及其多糖含量测定

Establishment of high performance liquid chromatography fingerprint and determination of polysaccharide content in Polygonatum kingianum from different origins

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中文摘要：目的探讨不同产地滇黄精高效液相色谱法(HPLC)指纹图谱的建立及其多糖含量的测定.方法取贵州(剑河、安顺、天柱、铜仁、赤水、贵阳及纳雍)、广西百色及四川内江来源的9个批次滇黄精药材各1 g制备供试品溶液,采用DiKMA Platisll ODS C18色谱柱(流动相为乙腈-0.05％磷酸水溶液、梯度洗脱,流速1 mL/min,柱温35℃,进样量20 µL,检测波长203 nm)建立滇黄精HPLC指纹图谱,并采用相似度评价、主成分分析及聚类分析等方法评价不同产地滇黄精的质量;采用蒽酮-硫酸法测定各批次滇黄精的多糖含量.结果建立了 9批滇黄精的HPLC指纹图谱,共确认12个共有峰;9批不同产地的滇黄精多糖含量均大于7％,含量顺序为贵州剑河＞贵州安顺＞贵州天柱＞贵州铜仁＞贵州赤水＞贵州贵阳＞贵州纳雍＞广西百色＞四川内江;相似度结果显示,四川内江、贵州贵阳及广西百色的相似度分别为0.762、0.809及0.846,贵州其余地区滇黄精的相似度≥0.853;主成分分析结果显示共得到3个主成分因子,聚类分析结果表明滇黄精被分为3类,且滇黄精多糖含量≥7.16％.结论本研究建立的滇黄精HPLC指纹图谱及黄精多糖成分含量的测定方法简便可行,准确可靠.

外文摘要：Objective To investigate establishment of high performance liquid chromatography(HPLC)fingerprints and determination of polysaccharides content in Polygonatum kingianum from different origins.Methods Nine batches of Polygonatum kingianum(1 g)from Guizhou(Jianhe,Anshun,Tianzhu,Tongren,Chishui,Guiyang,and Nayong),Baise Guangxi,and Neijiang Sichuan sources were taken to prepare the test solution(1 g from each origin).The separation was performed on a DiKMA Platisll ODS C18(250 mm × 4.6 mum,5 μm)column(with acetonitrile-0.05％phosphoric acid aqueous solution as mobile phase in gradient elution at a flow rate of 1 mL/min and a column temperature of 35 ℃ with an injection volume of 20 μL and a detection wavelength of 203 nm)to establish HPLC fingerprints of Polygonatum kingianum extracts;the quality of Polygonatum kingianum from different origins was evaluated by similarity evaluation,principal component analysis and cluster analysis;the content of polysaccharides of Polygonatum kingianum from each batch was determined by anthrone-sulfuric acid method.Results HPLC fingerprints were established for nine batches of Polygonatum kingianum,and a total of 12 common peaks were identified.The polysaccharide content in all nine batches from different sources exceeded 7％.Among them,the descending order of polysaccharide content was as follows:Jianhe Guizhou＞Anshun Guizhou＞Tianzhu Guizhou＞Tongren Guizhou＞Chishui Guizhou＞Nayong Guizhou＞Baise Guangxi＞Neijiang Sichuan.The similarity analysis results indicated that the similarities between Neijiang Sichuan,Guiyang Guizhou and Baise Guangxi were 0.762,0.809,and 0.846,respectively;while the similarity of Polygonatum kingianum between other areas in Guizhou province were ≥ 0.853.Furthermore,three principal component factors were obtained using principal component analysis.Cluster analysis revealed that Polygonatum kingianum could be categorized into three distinct groups and content of polysaccharides of Polygonatum kingianum were ≥7.16％.Conclusion The method of HPLC fingerprints and determination of polysaccharides content in Polygonatum kingianum is simple,feasible,accurate,and reliable.

外文关键词：

cluster analysisprincipal component analysisPolygonatum kingianumfingerprinthigh performance liquid chromatographyultraviolet spectrophotometry

作者：

马琴、郭思潍、杜强、杨国海、王才武、周雪、吴林菁

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作者单位：

贵州医科大学药学院天然药物资源优效利用重点实验室&贵州省高等学校天然药理与成药性评价重点实验室,贵州贵阳 561113

贵州省农业科学院,贵州贵阳 550025

天柱县三方田种植专业合作社,贵州天柱 556608

关键词：

聚类分析主成分分析滇黄精指纹图谱高效液相色谱法紫外分光光度法

基金：

贵州省科技计划项目贵州省科技计划项目贵州省科技计划项目贵州省科技创新基地贵州省高层次创新型人才百层次人才

项目编号：

黔科合基础-ZK[2022]一般389黔科合支撑[2020]4Y104黔科合支撑[2023]一般046黔科合中引地[2023]003黔科合平台人才-GCC[2023]048

出版年：

2024

DOI：

10.19367/j.cnki.2096-8388.2024.03.009

贵州医科大学学报

贵阳医学院

贵州医科大学学报

CSTPCD

影响因子：0.827

ISSN：2096-8388

年,卷(期)：2024.49(3)

参考文献量34