Objective To evaluate the frequencies of immune cells of patients with multiple sclerosis(MS)and its animal model so as to explore the immune status of patients with MS.Methods Twenty-eight patients with MS and 25 healthy controls(HCs)were recruited.The routine parameters of peripheral blood were collected.The frequencies of lymphocyte subsets were measured by flowcytometric assay.Female wild-type(WT)mice(8-10 weeks old,C57BL/6 background)were randomly divided into the control group by subcutaneously injection of PBS and EAE group with myelin oligodendroglycoprotein 35-55(MOG35-55)subcutaneous injection,with 5 mice in each group.Fifteen days later,brains,spinal cords and spleens were collected.The frequencies of relevant immune cells in brain spinal cord and spleen were evaluated using flow cytometry.Results Compared with HCs,the frequencies of monocytes(Mono),and eosinophils(Eos)in MS group were obviously down-regulated(P＜0.05)and so was T cells(P＜0.01),while the frequency of B cells of MS group was obviously up-regulated(P＜0.01).Compared with the control group,the frequencies of lymphocytes(CD45+),macrophages(CD45+,CD11b+)and CD4+T were significantly up-regulated,but frequencies of CD8+T were significantly reduced in the brain and spinal cord of EAE group(P＜0.01);furthermore,the frequencies of macrophages(CD11b+,F4/80+)and neutrophils(CD11b+,GR-1+)were apparently up-regulated and the frequencies of CD4+T in spleens were apparently down-regulated in EAE group(P＜0.01).Conclusion Some disorders of the immune cells may occur in the patients with MS and animal models.
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