首页|多发性硬化症患者及动物模型相关免疫细胞比例的变化

多发性硬化症患者及动物模型相关免疫细胞比例的变化

Variation of the frequencies of immune cells of patients with multiple sclerosis and its animal model

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目的 探究多发性硬化症(MS)患者及动物模型相关免疫细胞比例变化.方法 选取28例MS患者作为MS组,同期25例健康体检者作为对照组(HCs组),收集两组受检者外周血血常规参数,流式细胞术检测其淋巴细胞亚群百分比;采用髓鞘少突胶质细胞糖蛋白片段35-55(MOG35.55)在C57BL/6小鼠身上构建实验性自身免疫性脑脊髓膜炎(EAE)模型,10只小鼠均分为对照组(control组,皮下注射等量PBS)与EAE组(注射MOG35-55),于造模后第15天取2组小鼠大脑、脊髓和脾脏,流式细胞术检测大脑、脊髓和脾脏相关免疫细胞百分比.结果 MS组患者外周血单核细胞(Mono)和嗜酸性粒细胞(Eos)比例较HCs组显著下调(P＜0.05),B细胞比例显著上调(P＜0.01),T细胞比例显著下调(P＜0.01);EAE组小鼠较control组小鼠大脑和脊髓中淋巴细胞(CD45+)、巨噬细胞(CD45+、CD11b+)、CD4+T比例显著升高(P＜0.01),CD8+T比例显著下调(P＜0.01);脾脏中巨噬细胞(CD11b+、F4/80+)、中性粒细胞((CD11b+、GR-1+)比例显著上调(P＜0.001),CD4+T比例显著下调(P＜0.01).结论 MS患者及动物模型机体存在免疫细胞比例失调现象.
Objective To evaluate the frequencies of immune cells of patients with multiple sclerosis(MS)and its animal model so as to explore the immune status of patients with MS.Methods Twenty-eight patients with MS and 25 healthy controls(HCs)were recruited.The routine parameters of peripheral blood were collected.The frequencies of lymphocyte subsets were measured by flowcytometric assay.Female wild-type(WT)mice(8-10 weeks old,C57BL/6 background)were randomly divided into the control group by subcutaneously injection of PBS and EAE group with myelin oligodendroglycoprotein 35-55(MOG35-55)subcutaneous injection,with 5 mice in each group.Fifteen days later,brains,spinal cords and spleens were collected.The frequencies of relevant immune cells in brain spinal cord and spleen were evaluated using flow cytometry.Results Compared with HCs,the frequencies of monocytes(Mono),and eosinophils(Eos)in MS group were obviously down-regulated(P＜0.05)and so was T cells(P＜0.01),while the frequency of B cells of MS group was obviously up-regulated(P＜0.01).Compared with the control group,the frequencies of lymphocytes(CD45+),macrophages(CD45+,CD11b+)and CD4+T were significantly up-regulated,but frequencies of CD8+T were significantly reduced in the brain and spinal cord of EAE group(P＜0.01);furthermore,the frequencies of macrophages(CD11b+,F4/80+)and neutrophils(CD11b+,GR-1+)were apparently up-regulated and the frequencies of CD4+T in spleens were apparently down-regulated in EAE group(P＜0.01).Conclusion Some disorders of the immune cells may occur in the patients with MS and animal models.

multiple sclerosisanimal modelexperimental autoimmune encephalomyelitislymphocyte subsetsimmune cells disordersblood-brain barrier

何菁菁、晏世荣、吴通前、杨秀兰、沈雪、余芳

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贵州医科大学附属医院临床检验中心,贵州贵阳 550004

贵州医科大学医学检验学院,贵州贵阳 550004

贵州医科大学附属医院临床研究中心,贵州贵阳 550004

多发性硬化症 动物模型 实验性自身免疫性脑脊髓膜炎 淋巴细胞亚群 免疫细胞比例失调 血脑屏障

国家自然科学基金国家自然科学基金国家自然科学基金贵州省基础研究计划项目贵州省基础研究计划项目贵州省卫健委科学技术基金

817602948226032482201968黔科合基础-ZK[2023]一般394黔科合基础-ZK[2023]一般398gzwkj2022-064

2024

10.19367/j.cnki.2096-8388.2024.04.006

贵州医科大学学报
贵阳医学院

贵州医科大学学报

CSTPCD
影响因子：0.827
ISSN：2096-8388
年,卷(期)：2024.49(4)
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