Objective To investigate the mechanism of Changbing decoction in combating ulcerative colitis(UC)via regulating phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/nuclear factor-kappa B(NF-κB)signaling pathway.Methods Forty-two C57BL/6 mice were randomly divided into normal control group(n=6,Control group,freely drinking deionized water),model group(n=12,DSS group),mesalazine group(n=12,MES group)and Changbing decoction group(n=12,CBD group).Model,DSS and CBD groups were given with 3％dextran sulfate sodium(DSS)for 7 days to establish UC model.At the same time,DSS and CBD groups were intragastrically administrated with mesalazine and Changbing decoction,respectively,while control and model groups were intragastrically administrated with equal volume of deionized water.Mouse body mass changes were observed during the experiment.Mouse disease activity index(DAI)was evaluated.After the completion of modeling,the mice were anesthetized and euthanized.Colon lengths were measured.The contents and mucosa of the colons were observed.Hematoxylin eosin(HE)staining was used to observe the histopathological changes of mouse colons in each group.Enzyme-linked immunosorbent assay(ELISA)was performed to detect the levels of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in mouse colon tissue homogenates in each group.Western blot was used to detect phosphorylated-PI3K(p-PI3K p85),phosphorylated-AKT1(p-AKT1),and phosphorylated-NF-κB(p-NF-κB p65)in mouse colon tissues in each group.Real-time fluorescence quantitative PCR(qRT-PCT)was used to detect mRNA expression levels of PI3K,AKT,and NF-κB in mouse colon tissues.Results When compared with DSS group,CBD group exhibited alleviated UC symptoms,improved fecal traits and stool blood,reduced colon mucosal inflammatory infiltration,alleviated body mass,decreased DAI score,colon lengths close to normal,decreased IL-6 and TNF-α expressions in colon tissue homogenates after treatment(P＜0.05).The protein and mRNA expressions of p-PI3K-p85,p-AKT1,and p-NF-κB P65 were significantly decreased(P＜0.05).Conclusion Changbing decoction can alleviate the symptoms and colon mucosal injury of UC mice,and its mechanism may be related to regulating the expression of PI3K/AKT/NF-κB signaling pathway and reducing the expression of inflammatory factors in colon tissues.
维普
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