Objective To investigate the effect of aconite-containing serum on invasion and migration of human liver cancer cells and its mechanism.Methods SD rats were orally administered with raw aconite decoction at a concentration of 50 g/L and 0.9％physiological saline,respectively.After one week,the aconite-containing serum and blank serum were obtained.Liver cancer cell lines SK-Hep-1 and SMMC-7721 were treated with different concentrations of aconite-containing serum(5％,10％,15％,and 20％)to screen optimal aconite-containing serum concentration.SK Hep-1 and SMMC-7721 cells at logarithmic phase growth were used for CCK8 assay to detect the effect of aconite-containing serum on liver cancer cell proliferation.Flow cytometry was used to measure the effect of aconite-containing serum on liver cancer cell apoptosis.Transwell assay and wound healing assay were applied to evaluate the effects of different concentrations of aconite-containing serum on cell invasion and migration ability.Real time quantitative polymerase chain reaction(RT-qPCR)and Western blot were used to detect mRNA and protein expression levels of calmodulin 1(CDH1),CDH2,and nuclear factor-κB(NF-κB).Enzyme-linked immunosorbent assay(ELISA)was performed to measure content changes of tumor necrosis factor-α(TNF-α),transforming growth factor-β1(TGF-β1)and interleukin-10(IL-10).Results Aconite-containing serum significantly inhibited the proliferation of liver cancer cells,increased their apoptosis rates and reduced cell migration and invasion abilities.These effects were intensified with the increase of the concentration of aconite-containing serum.Aconite-containing serum increased the protein and mRNA expressions of CDH1,while decreased the protein and mRNA expressions of CDH2 and NF-κB.Aconite-containing serum also attenuated TNF-α and TGF-β,while augmented IL-10 in a dose-dependent manner(P＜0.05).Conclusion Aconite-containing serum inhibits the proliferation,migrationand invasion ability of human liver cancer cells,promotes their apoptosis.Its mechanism may be related to regulating CDH1,CDH2,and NF-κB.
关键词
附子/含药血清/肝癌细胞/侵袭/迁移/增殖能力
Key words
aconite/aconite-containing serum/liver cancer cells/invasion/migration/proliferative ability
维普
万方数据