Objective To investigate the effect of long non-coding ribonucleic deleted in llymphocytic leukemia 2(LncRNA DLEU2)on the growth and proliferation of human colon cancer SW1116 cell by targeting micrornas(miR)-30a-5p/integrin β3(CD61).Methods Human colon cancer cell strain SW1116 was cultured and randomly divided into:si-NC group,si-LncRNA DLEU2 group,miR-NC negative control group,miR-30a-5p mimics group,miR-30a-5p NC inhibitor+si-LncRNA DLEU2 group,miR-30a-5p Inhibitor+si-LncRNA DLEU2 group;meanwhile,un-transfected cells were set as blank group.After transfection 48h,the transfection efficiency of each group was detected by fluorescence microscope,and MTT assay was used to detect the proliferation activity of cells in each group;and the LncRNA DLEU2 and miR-30a-5p expressions,the mRNA and protein expressions of CD61,CyclinD1,c-Myc in each group were detected by real-time quantitative polymerase chain reaction(RT-qPCR)and western blotting;dual luciferase reporter assay and RT-qPCR were used to detect the targeting relationship between LncRNA DLEU2 and miR-30a-5p,miR-30a-5p,and CD61.The effect of LncRNA DLEU2 on tumor growth in vivo was evaluated in xenograft tumor model of male thymus BALB/C.Results The transfection efficiency of si-NC group,si-LncRNA DLEU2 group,miR-NC group,miR-30a-5p mimics group,NC inhibitor+si-LncRNA DLEU2 group,and miR-30a-5p inhibitor+si-LncRNA DLEU2 group was more than 85％.Compared with blank group,si-NC group,the proliferation inhibition rates of si-LncRNA DLEU2 group,NC inhibitor+si-LncRNA DLEU2 group increased(P＜0.05),the expression of DLEU2 decreased(P＜0.05),and the expression of miR-30a-5p increased(P＜0.05);the mRNA and protein expressions of CD61,CyclinD1,c-Myc decreased(P＜0.05).Compared with NC inhibitor+si-LncRNA DLEU2 group,the proliferation inhibition rate of miR-30a-5p inhibitor+si-LncRNA DLEU2 group decreased(P＜0.05),and the expression of miR-30a-5p decreased,while the expressions of CD61,CyclinD1,c-Myc mRNA and protein increased(P＜0.05).Compared with miR-NC group,the expression of miR-30a-5p and proliferation inhibition rate increased in miR-30a-5p mimics group,while the mRNA and protein expressions of CD61,CyclinD1 and c-Myc decreased(P＜0.05).LncRNA DLEU2 can directly target miR-30a-5p and miR-30a-5p can directly target CD61.In addition,inhibition of LncRNA DLEU2 expression could reduce BALB/c tumor growth in nude mice(P＜0.05).Conclusion Silencing LncRNA DLEU2 could inhibit the growth and proliferation of human colon cancer cell strain SW1116,which may be related to the inhibition of CD61 expression and the inhibition of CyclinD1 and c-Myc expression by targeting miR-30a-5p.
long non-coding ribose nucleic acid DLEU2micro ribonucleic acid-30a-5pcolon cancercellproliferation
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