Objective To study the main genotypes distribution and drug resistance of Mycobacterium tuberculosis(MTB)in Anshun region in Guizhou province.Methods RD 105 gene deletion and duplex PCR were used to genotype 152 strain MTB clinical isolates of in Anshun region.Drug resistance was analyzed by drug sensitivity test.Gene sequencing technique was used to sequence RNA polymerase β subunit(rpoB),an indicator of Beijing rifampicin resistant MTB.Results Among 152 strain MTB isolates,there were 122 strains with Beijing genotype(80.26％)and 30 strains with non-Beijing genotype(19.74％).There were 108 strains sensitive to anti-tuberculosis drugs and 44 strains resistant to 16 anti-tuberculosis drugs.Among 44 MTB resistant strains,31 strains were resistant to rifampicin,accounting for 70.45％.Beijing genotype MTB had a significantly higher resistance rate to rifampicin than non-Beijing genotype MTB(x2=6.896,P=0.009).There were 13 non-rifampicin resistant tuberculosis strains,accounting for 29.55％of the drug-resistant tuberculosis strains.The sequencing results of the rpoB gene for rifampicin resistance in Beijing genotype MTB showed that there were 15 strains with relevant site mutations in rpoB,among which,7 stains had the most significant mutation site was Ser450Leu in rpoB,accounting for 46.67％(7/15).Conclusions The prevalent genotype of MTB in Anshun region is Beijing genotype.The main drug-resistance type is rifampicin resistance.The mutation site of the resistance gene rpoB is Ser450Leu.
NETL
NSTL
万方数据
