Objective To explore the mechanism of long non-coding RNA potassium voltage-gated channel subfamily Q member 1 opposite strand/antisense transcript 1 ( LncRNA KCNQ1 OT1 )targeting miR-9-5p on oral squamous cell carcinoma cell adhesion and activity. Methods SCC25 cells of oral cancer were transfected with LncRNA KCNQ1OT1 negative control,LncRNA KCNQ1OT1 siRNA,miR-9-5p negative control,miR-9-5p siRNA,and LncRNA KCNQ1OT1 siRNA and miR-9-5p siRNA,respectively. They were divided as KCNQ1OT1-NC ( K1-NC ) group,KCNQ1OT1 ( K1 ) group,miR-9-5p-NC (m-NC) group,miR-9-5p (m9-5p) group,KCNQ1OT1+miR-9-5p(K1+m9-5p)group,and untransfected cells were divided as oral cancer group. After transfection,the levels of LncRNA KCNQ1OT1 and miR-9-5p were determined by RT-PCR. Absorbance ( OD) and apoptosis rate were measured by MTT and flow cytometry,respectively;and the expressions of CD44S and CD44V5 in cells were measured by Western blot,and the targeting relationship between LncRNA KCNQ1OT1 and miR-9-5p was detected by dual lucifluciferase reports. GraphPadPrism 8. 0software was used to analyze the statistical differences. Results The LncRNA KCNQ1OT1 level in K1 group was lower than that in oral cancer group ( P<0.05 ),and the miR-9-5p level in miR-9-5p group was lower than that in oral cancer group (P<0.05). Compared with oral cancer group,OD value,CD44S,and CD44V5 in K1,miR-9-5p,and K1+miR-9-5p groups decreased,and apoptosis rate increased ( P<0.05 ),and the effect of the treatment for K1+miR-9-5 p group was the most significant ( P<0.05 ) . Dual luciferase reports showed that miR-9-5 p was the target gene of LncRNA KCNQ1OT1.Conclusions LncRNA KCNQ1OT1 may regulate the expression of miR-9-5p to reduce the adhesion,inhibit the proliferation of oral squamous cell carcinoma cells and induce the apoptosis of oral squamous cell carcinoma cells.
维普
万方数据