摘要
目的 研究金骨莲胶囊的抗炎镇痛作用及各组方配伍的抗炎镇痛内在规律.方法 108只健康昆明种小鼠随机分为对照组(CON组,0.5％羧甲基纤维素钠)、阳性药组(ASPL组,阿司匹林)、全方组(QF组,金骨莲胶囊)、主药组(A组,金铁锁)、辅药组(B组,透骨香、汉桃叶、八角枫)、引药组(C组,大血藤)、主+辅组(A+B组)、主+引组(A+C组)及辅+引组(B+C组)共9组,采用小鼠耳廓肿胀实验评价金骨莲胶囊的抗炎作用,采用醋酸致小鼠扭体反应实验评价其镇痛作用,并结合正交t值法探讨金骨莲方的组方配伍内在规律.结果 与CON相比,ASPL组、QF组、A组、B组、C组、A+B、A+C组以及B+C组的小鼠耳廓肿胀抑制率和扭体抑制率均有不同程度的升高(P＜0.05或P＜0.01),且QF组耳廓肿胀抑制率和扭体抑制率最高;正交t值表显示,QF组能显著抑制小鼠耳廓肿胀度(P＜0.01)和小鼠扭体次数(P＜0.05),交互作用明显.结论 金骨莲胶囊具有显著的抗炎镇痛作用,拆方研究表明该方存在配伍增强效应.
Abstract
Objective To explore the anti-inflammatory and analgesic effects of Jingulian capsules and the inherent laws of anti-inflammatory and analgesic effects of formula compatibility.Methods A total of 108 healthy Kunming mice were randomly divided into 9 groups:control group(CON group,0.5％Carboxymethylcellulose sodium),positive drug group(ASPL group,aspirin),whole prescription group(QF group,Jingulian Capsules),main drug group(A group,Psammosilene tunicoides),auxiliary drug group(B group,Gaultheriae Yunnanensis Herba seu Radix,Schefflerae Leucanthae Ramulus et Folium and Alangii Radix),lead drug group(C group,Sargentodoxae Caulis),main+auxiliary group(A+B group),main+lead group(A+C group),and auxiliary+lead group(B+C group).Mouse ear swelling experiment was performed to evaluate anti-inflammatory effect of Jingulian Capsule.Analgesic effect of Jingulian was evaluated by acetic acid-induced writhing body reaction experiment in mice.The inherent compatibility law among each formula in Jingulian prescription were discussed by orthogonal t-value method.Results When compared to CON group,the inhibition rates of mouse ear swelling and torsion were both increased to varying degrees in ASPL,QF,A,B,C,A+B,A+C,and B+C groups(P＜0.05 or P＜0.01).QF group had the highest inhibition rates for ear swelling and torsion.The orthogonal t-value table showed that QF group could significantly inhibit the degree of mouse ear swelling(P＜0.01)and the number of mouse twisting movements(P＜0.05)with a significant interaction effect.Conclusion Jingulian capsule has significant anti-inflammatory and analgesic effects,and disassembled formulas show that the formulas have a compatibility enhancement effect.
维普
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