Tanshinone ⅡA promotes the repair of spinal cord injury by regulating astrocyte phenotype polarization
Objective To observe the effect of tanshinone ⅡA(TⅡA)on astrocyte phenotype polarization in spinal cord tissue in a rat spinal cord injury(SCI)model and motor function.Methods Ninety female SD rats were randomly divided into sham group,model group,TⅡA group,astrocyte inhibitor group(LAA group)and TⅡA+astrocyte inhibitor group(TⅡA+LAA group).Sham group was bit off T8-T10 laminae(without damaging dura mater).The last 4 groups of rats were used to create a spinal cord transection injury model,and the last 3 groups of rats were injected with corresponding drugs at the site of injury.After successful modeling,behavioral observations and Basso-Beattie-Bresnahan(BBB)locomotor rating scale were performed on the 3rd,7th,14th,21th,28th,and 56th postoperative days,respectively.The injured spinal cord tissues were collected and subjected to immunohistochemical staining(IHC)and immunofluorescent staining(IF),hematoxylin & eosin(H&E)staining and Nissl staining to observe histological changes in the spinal cords.qRT-PCR was used to detect C3 expression of A1 astrocyte marker,S100A10 expression of A2 astrocyte marker,glial cell marker GFAP and neuronal marker NF200 in each group of cells.Western blot was used to detect the protein expression of C3,S100A10,and GFAP in each group of cells.Results When compared to model group,TⅡA group had increased BBB score,the elevated mRNA and protein expressions of S100A10,NF200 and GFAP as well as reduced C3 mRNA and protein expressions(P＜0.05).There was no significant difference in Nissl body number between model and TⅡA groups.TⅡA improved LAA-mediated proliferation inhibition of astrocytes and activated reactive astrocytes.When compared to LAA group,TⅡA group had elevated S100A10 protein expression(P＜0.001).When compared to LAA group,TⅡA+LAA group showed an increase in S100A10 protein expression(P＜0.000 1).Conclusion Intraperitoneal injection of TⅡA can improve the motor function of rats with SCI.Its mechanism may be related to regulating the phenotype of reactive astrocytes,promoting the polarization of A2 astrocytes and reducing the pathological injury of spinal cord.
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