Objective To investigate the distribution of carbapenem-resistant Enterobacteriaceae(CRE)in intensive care unit(ICU)during hospitalization,examine and analyze CRE-related genes.Methods Rectal swabs,throat swabs,and groin swabs were collected.Broth cultivation was used amplify the samples from above swabs and screen CRE positive cases.Automatic drug sensitivity analyzer was used for in vitro drug sensitivity testing.PCR was applied to detect CRE genes.Multi-locus sequence typing(MLST)and Enterobacterial repetitive intergenic consensus(ERIC)PCR were used to determine homologous relationship.Results The positive rates of CRE in rectal swabs,throat swabs,and groin swabs were 12.5％,5.0％,and 4.2％respectively.The main CRE strains were Klebsiella pneumoniae(82.6％),Escherichia coli(9.8％),and Enterobacter aerogenes(4.3％).CRE strains exhibited good sensitivity to tigecycline,cefotaxime,and avibactam(＞90％).A total of 92 CRE strains were detected,and bla KPC-2 subtype was predominant,accounting for 91％.MLST typing results indicated that the majority of carbapenem-resistant K.pneumoniae(CRKP)belonged to ST11 type(69.7％),followed by ST15 type(28.9％).ERIC-PCR analysis demonstrated that most K.pneumoniae strains belonged to type A(69.7％)or type B(28.9％).Conclusion CRKP of bla KPC-2 subtype is a major strain found by active screening of CRE in ICU inpatients.MLST typing and cluster analysis of ERIC-PCR amplification reveal the spread of two clonal strains.
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