小肽GLV2参与调控拟南芥叶片形态建成
Peptide GLV2 is involved in regulating leaf morphogenesis in Arabidopsis
李佳璇 1杨佳圆 2吴良凡 3和黎明 3黎家 4李小鹏1
作者信息
- 1. 广州大学生命科学学院,广东广州 510006
- 2. 成都市盐道街小学528校区,四川成都 610011
- 3. 兰州大学生命科学学院,甘肃兰州 730030
- 4. 广州大学生命科学学院,广东广州 510006;兰州大学生命科学学院,甘肃兰州 730030
- 折叠
摘要
在植物中,小肽激素在调控植株生长发育及环境适应等过程中发挥着不可替代的作用,其中,GLVs家族被证明参与根尖分生组织发育与维持、根向地性、侧根发生及植物抗病等过程,而该家族成员是否参与调控地上部分的生长发育尚不清楚.本研究从分析GLVs小肽基因家族的组织表达出发,发现部分GLVs在叶中有表达,其中,GLV2表达丰度最高.利用CRISPR-Cas9基因编辑技术,本研究获得了 glv2突变体并对其表型进行了分析.结果显示,相较于野生型,glv2突变体莲座叶展现出叶片尖端及两侧边缘下卷、叶片狭长的表型.组织切片和扫描电镜观察表明,glv2叶片卷曲主要由海绵组织细胞发育异常造成.现有研究认为,小肽GLVs家族主要作为受体激酶RGIs家族的配体发挥功能.本研究通过表达模式分析发现,大部分RGls家族成员在叶中有表达,并且RGls家族五重缺失突变体展现glv2类似的叶片形态表型.进一步的qRT-PCR分析显示,glv2叶片中生长素相关基因出现上调表达,暗示着glv2中生长素信号被一定程度地激活,进而造成叶片形态变化.综上,本研究证明GLV2可能被RGIs感知后,通过调控生长素信号影响植物叶肉细胞发育,进而调控叶片形态建成.
Abstract
Peptide hormones play indispensable roles in regulating plant growth,development,and environmental adaptation.GLVs peptide family has been demonstrated to participate in regulating va-rious processes such as root meristem development,root gravitropism,lateral root initiation,and plant immunity.However,it remains unclear whether members of GLVs family are also involved in regula-ting the development of aerial parts of a plant.We examined the expression patterns of all 11 GLV genes,and found that some members are expressed in leaves,with GLV2 showing more expression than other GLVs.Using a CRISPR-Cas9 gene editing approach,we generated a glv2 mutant.Com-pared to the wild type,glv2 exhibited curled and elongated leaf phenotypes.Detailed examinations via histological sections and scanning electron microscopy analyses revealed that leaf curling in the glv2 mutant primarily resulted from the abnormal development of the spongy tissues.Existing researches demonstrate that GLV family members act primarily as the ligands of the receptor kinase family,RGIs.We also found that most RGI family members are expressed in leaves.A quintuple mutant of RGIs exhibited abnormal leaf curl phenotype similar to glv2.qRT-PCR analysis identified a number of auxin-related genes which are up-regulated in glv2 leaves,suggesting the potential activation of auxin signaling as a contributing factor to the altered leaf morphology in the glv2 mutant.In summary,our results suggest that GLV2,likely perceived by RGIs,modulates plant leaf mesophyll cell development by regulating auxin signaling,thereby influencing leaf morphogenesis.
关键词
植物小肽/GLVs/RGIs/生长素/叶片形态Key words
peptides/GLVs/RGIs/auxin/leaf morphogenesis引用本文复制引用
基金项目
教育部重点实验室开放基金(lzujbky-2022-kb03)
广州市基础与应用基础研究专题青年博士"启航"项目(2024A0453252)
出版年
2024
NETL
NSTL
万方数据