首页|双重培养体系中AM真菌孢子的种类鉴定

双重培养体系中AM真菌孢子的种类鉴定

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利用AM真菌单孢与烟草Ri-TDNA转型根建立了离体双重培养体系,该体系产生了大量孢子,通过孢子形态特征和18S rDNA AML1-AML2区序列的系统发育分析进行种属鉴定,鉴定出该菌种为珠状巨孢囊霉Gigaspora margarita.
Species Identification of AM Fungal Spores from Dual Culture System
Dual culture system between the arbuscular mycorrhizal fungi and hairy root of tobacco was established and pure AM fungi spores were obtained. Morphological method and Nested PCR technology were used to identify its regenerated spores. The results show that the spores were identified as Gigaspora margarita by the following two methods, morphological identification and molecular phylogenetic analysis of AML1-AML2 region sequence of 18S rDNA.

hairy root of tobaccoArbuscular mycorrhizal fungimolecular phylogenetic analysis

骆礼华、姚刘斌、江龙

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贵州大学 生命科学学院,贵州 贵阳550025

贵州大学 贵州省烟草品质研究重点实验室,贵州 贵阳550025

烟草毛状根 AM真菌 分子系统发育分析

国家科技支撑项目资助贵州省科学技术基金资助

2015BAD04B0204黔科合J字[2009]2282号

2017

贵州大学学报(自然科学版)
贵州大学

贵州大学学报(自然科学版)

CSTPCD
影响因子:0.396
ISSN:1000-5269
年,卷(期):2017.34(5)
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