Screening of a Straw Degradation Strain and Study on Its Degradation Effect
At present,crop straw returning to the field is faced with some problems,such as long time,slow maturity and low humus transformation,which can be effectively solved by adding straw degradation bacteria in the process of returning crop straw to the field.In this study,using rotten straw and soil as bacterial sources,a strain TP-125 with good ability to degrade lignocellulose was isolated and screened.Combined with morphology and molecular biology,the strain was identified as Bacillus subtilis,and the best exponential growth period of TP-125 was 22 h.Through enzyme-producing medium fermentation,the filter paper enzyme activity of strain TP-125 reached the highest on the 5th day,which was 16.76 U/mL.The activity of Endo-β-glucanase reached the highest level on the 6th day,which was 22.16 U/mL.The fermentation broth of the dominant degrading bacteria TP-125 was compared with three kinds of ripening agents sold on the market,and inoculated into rape,corn,pepper and barley straw,buried in the soil for degradation and maturity.After 14 days of maturity,the results show that the comprehensive straw degradation effect of TP-125 is stable and not weaker than the other three maturity agents of the market,among which the degradation effect of Coix seed straw is the best,and the degradation rates of cellulose,lignin,and hemicellulose in rapeseed straw is 28.30%,30.80%,and 53.40%,respectively,the carbon content of humus is the highest,reaching 328.22 g/kg.To sum up,strain TP-125 has a good application potential in the degradation of crop straw and can be used as a candidate strain for crop straw decomposition agent.
crop strawcellulolytic bacteriaisolation and identificationbacillus subtilis
万方数据
维普
