Determination of Flavonoids from Miao Medicine Asplenium varians Wall. ex Hook. et Grev. with Ultraviolet Spectrophotometry
In order to scientific and reasonable use Asplenium varians Wall. ex Hook. et Grev. , ultrasonic-assisted was used to extract flavonoids in Asplenium varians Wall. ex Hook. et Grev. The concentration of flavonoids in Asplenium varians Wall. ex Hook. et Grev. was determined with ultraviolet spectrophotometry. The egression equation of rutin standard curve was A=0. 0133 C+0. 0042 , R=0. 9983 , the relative standard deviation was 0. 60% and the average recovery was 99. 7%, the detection of total flavonoids was at 510 nm wavelength and its level was 11. 56 mg/g ( n=5 ) , the results provided scientific basis for the utilization of Asplenium varians Wall. ex Hook. et Grev.
Asplenium varians Wall. ex Hook. et Grev.flavonoidsultraviolet spectrophotometry