GC-MS Analysis and in Vitro Study on Pharmacological Activity of Rosemary Essential Oil
The rosemary essential oil was analyzed by gas chromatography-mass spectrometry(GC-MS),and the relative content of each component was calculated.The Oxford cup method was used to evaluate its in vitro antibacterial effect against Escherichia coli,Staphylococcus aureus and Candida albicans,lipopolysaccharide(LPS)was used to induce RAW264.7 to establish inflammatory model,and used real-time fluorescence quantitative PCR(qPCR)to detect interleukin-6 and tumor necrosis factor TNF-α in RAW264.7,the relative expression level of mRNA was used to investigate its anti-inflammatory effect.28 volatile components were identified,of which the highest content of eucalyptol was 30.62%.The results of antibacterial experiment showed that rosemary essential oil had certain antibacterial effect on three strains,and the antibacterial effect on Escherichia coli was the best.Rosemary essential oil can significantly inhibit TNF-α,IL-6 the relative expression of RAW264.7 in mRNA,and it was dose dependent.
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