To study the chemical constituents from cultures of Hebeloma testaceum,the cultures were first extracted with ethyl acetate.Subsequently,the compounds were isolated using silica gel and Sephadex LH-20.Their structures were elucidated by means of spectral analysis.Consult the literature,six compounds were isolated and identified as taxifolin(1),β-sitosterol(2),methyl linoleate(3),dibutyl phthalate(4),9(Z),12(Z)-octadecanedioenoic acid(5),2,3-butanediol(6).Range of compounds 1 to 6 were isolated from this species for the first time.Among them,taxifolin(1)is a flavonoid with biological and pharmacological activities such as antioxidant,anti-inflammatory,antitumor and antibacterial activities.This will provide a scientific basis and reference for the rational development and utilization of Hebeloma testaceum in the future.
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