In this paper,a phenoxyacetic acid complex was synthesized with phenoxyacetic acid as the main ligand.The interaction between complex and calf thymus DNA(CT-DNA)and human serum albumin(HSA)was investigated using UV-Vis and fluorescence spectroscopy.The results indicated an increase in the viscosity of CT-DNA with increasing complex concentration,suggesting that complex binds to DNA in an intercalative manner.The binding constant between complex and CT-DNA Kb=1.43×103 L/mol with a binding site number n=1,while the quenching constant(Ksv)and quenching rate constant(Kq)for CT-DNA were determined to be 2.04×103 L/mol and 2.04×1011 L/mol·s-1,respectively.The complex also exhibited static quenching of the fluorescence intensity of HSA,with Ksv=9.49×108 L/mol·s-1,Kq=9.49×1016 L/mol·s-1,and Ka=5.53×109 L/mol,n=1.In summary,the complex exhibited a static quenching effect on the fluorescence intensity of CT-DNA and HSA.
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