Study on Cellulase-assisted Ultrasound Extraction on Polyphenols from Mulberry Leaves
To optimize the cellulase-assisted ultrasound extraction conditions for polyphenols from Mulberry Leaves,cellulase-assisted ultrasound extraction method was used to extract polyphenols from mulberry Leaves.The extraction rate of mulberry leaf polyphenols was selected as the index.The effects of enzymatic hydrolysis temperature,enzymatic hydrolysis time,mulberry leaf powder to liquid ratio and extraction time on the extraction rate of polyphenols from mulberry leaf were studied.The extraction process of polyphenols of mulberry Leaves was optimized via the orthogonal experiment based on the result of the single factor experiment.The result of orthogonal experiment showed that the influence of these factors on it was in order of extraction time,enzymatic hydrolysis temperature,mulberry leaf powder to liquid ratio,and enzymatic hydrolysis time.The optimal process conditions for cellulase assisted ultrasonic extraction of mulberry leaf polyphenols were as follows:enzymatic hydrolysis temperature was 50℃,enzymatic hydrolysis time was 25 min,mulberry leaf powder to liquid ratio was 1∶35 g/mL,and extraction time was 40 min.Cellulase-assisted ultrasound extraction method can significantly improve the extraction efficiency,and can be used for extracting polyphenols from mulberry leaves.
万方数据
维普
