Mechanism of Jiangtangjia granules in the treatment of diabetes mellitus type 2
Based on bioinformatics,network pharmacology and molecular docking,this paper explores the action mechanism of Jiangtangjia granules(JTJ)in the treatment of diabetes mellitus type 2(T2DM).The active com-pounds and action targets of JTJ were screened out by using TCMSP,Uniprot,swisstarget and SEA databases,and the data of pancreas islet samples from T2DM patients and non-T2DM patients were obtained from GEO database.The differentially expressed genes were screened out by using R language limma package,and the JTJ-T2DM com-mon targets were obtained.The JTJ-T2DM-target network map was drawn by Cytoscape 3.9.1,and the PPI net-work map of JTJ-T2DM common targets was produced and the key targets(Hubs)were obtained by using STRING database and cytoHubb plugin of Cytoscape 3.9.1.The DAVID platform was used to perform GO functional enrich-ment analysis and KEGG pathway enrichment analysis on JTJ-T2DM common targets.Differential expression of the common targets and ROC analysis were performed.Finally,molecular docking was carried out by using DS BIOVIA software.The results showed that 41 JTJ active compounds,909 drug targets,2952 T2DM targets,160 JTJ-T2DM crossover targets,and 12 Hubs were obtained.The results of GO and KEGG enrichment analysis showed that JTJ mainly involved in apoptosis and regulated MAPK,AGE-RAGE and other signaling pathways to treat T2DM.Dif-ferential expression results showed that MAPK14,PRKCZ and PTPN11 were significantly differentially expressed and had high diagnostic value.Molecular docking results showed that(+)-syringaresinol-O-beta-D-glucoside had the strongest affinity with LCK and PTPN11,and linarin had the strongest affinity with PRKCZ.This study has pre-liminarily revealed the action mechanism of Jiangtangjia granules in the treatment of diabetes mellitus type 2,and has provided a sufficient and reliable theoretical basis for its treatment of diabetes mellitus type 2.
Jiangtangjia granules(JTJ)diabetes mellitus type 2action mechanism
万方数据
维普
