Mechanism of Mahonia bealei(Fort.)Carr.regulating immunity in diabetic reti-nopathy based on bioinformatics and molecular docking
This study explored the relationship between diabetic retinopathy(DR),immunity and Mahonia bealei(Fort.)Carr.based on bioinformatics and molecular docking.The targets of Mahonia bealei were obtained from liter-ature and Symmap,Swisstarget and TCMSP databases.the GSE53257 and GSE60436 gene microarray datasets were obtained from GEO,and the GEO2R tool was applied to pre-process the data.The data was evaluated by PCA anal-ysis using the R package stats,and the R language CIBERSORT package was used for immune infiltration and WGCNA analysis.The differentially expressed genes obtained from the dataset,the targets of Mahonia bealei,and the immune-related genes obtained by WGCNA were intersected.The GeneMANIA database was used to predict the genes related to the intersection targets and construct the interaction network map of the key genes.GO functional annotation and KEGG pathway enrichment of the intersection genes were performed with the help of DAVID data-base.Finally,molecular docking was performed to clarify the main active compounds in Mahonia bealei.Eight Hub genes(CDK2、CDK1、CHEK1、TP53、CDK6、CCND1、TYMS and CDC45)were obtained.KEGG was mainly enriched in melanoma,glioma and cAMP signaling pathways.The proteins of seven of the Hub genes(CDK6,CHEK1,CDK2,CDK1,CCND1,TP53 and TYMS)were found to bind well to 11 compounds(quercetin,rhodopsin,berberine,isorh-amnetin,etc.)in Mahonia bealei by molecular docking.This study initially elucidated the relationship between im-munity,Mahonia bealei and DR through bioinformatics and molecular docking,and provided a theoretical basis for the clinical application of Mahonia bealei in the treatment of DR.
NETL
NSTL
万方数据
维普
