Mechanism of puerarin regulating ferroptosis in hepatocellular carcinoma
In this study we investigated the effect of puerarin on hepatocellular carcinoma by regulating ferropto-sis.Network pharmacology,bioinformatics and molecular docking methods were used to comprehensively analyze the common targets of puerarin,ferroptosis and hepatocellular carcinoma.The intersection targets were PTGS2,AKRv1C3,JUN,PLIN2,and ALB.Among them,four key targets(AKR1C3,ALB,PTGS2,JUN)could bind to puerar-in,and the differential expression results showed that there were significant differences.THPA verification showed that the expression results in normal human tissues and in cancer tissues were consistent.Clinical correlation analy-sis showed that all four targets had outstanding clinical significance.Survival analysis curve showed that AKR1C3 had significant survival significance in the ten-year survival prognosis analysis(P＜0.05),while ALB,PTGS2 and JUN had poor survival significance(P＞0.05).The ROC curve showed that the predictive ability of AKR1C3 had high accuracy(AUC=0.945,CI=0.922-0.968).Immune infiltration analysis showed that AKR1C3 expression was positively correlated with the infiltration of macrophages and other cells,while negatively correlated with CD4+T cells.In conclusion,puerarin may regulate ferroptosis in hepatocellular carcinoma by targeting AKR1C3 gene.
NETL
NSTL
万方数据
维普
