HPLC法测定绿豆衣中2种成分
Determination of two constituents in mung bean hull by HPLC
卢婷 1姚斌1
作者信息
- 1. 黔东南州食品药品检验检测中心,贵州 凯里 556000
- 折叠
摘要
目的:建立高效液相色谱法测定绿豆衣中牡荆素和异牡荆素含量.方法:采用高效液相色谱法(HPLC)测定.色谱柱为Thermo C18(4.6 mm×250 mm,5 μm),流动相为甲醇-0.5%磷酸(40∶60,V/V),流速为1.0 mL/min;检测波长为340 nm,进样体积10 μL.结果:牡荆素和异牡荆素质量浓度分别在10.0～80.0 μg/mL和2.00～30.0 μg/mL范围内与峰面积线性关系良好(r=0.9999 和r=0.9999);平均加样回收率分别为 98.89%(RSD=0.21%)和 99.39%(RSD=0.26%).结论:建立的HPLC方法简便、准确、灵敏度高、专属性强,可用于绿豆衣中牡荆素和异牡荆素的含量测定.
Abstract
In this study we established an HPLC method for the content determination of vitexin and isovitexin in mung bean hull.The Thermo C18 column(4.6 mm×250 mm,5 μm)was adopted,the mobile phase was methanol-0.5%phosphoric acid(40∶60,V/V),the flow rate was 1.0 mL/min,the detection wavelength was 340 nm,and the injection volume was 10 μL.Vitexin and isovitexin had good linear relationship in the concentration ranges of 10.0-80.0 μg/mL and 2.00-30.0 μg/mL(r=0.9999),the average spiked recoveries were 98.89%and 99.39%,and the RSD were 0.21%and 0.26%.The established HPLC method is simple,accurate,sensitive and specific,and can be used for the content determination of vitexin and isovitexin in mung bean hull.
关键词
绿豆衣/牡荆素/异牡荆素/含量测定/高效液相色谱法Key words
mung bean hull/vitexin/isovitexin/content determination/HPLC引用本文复制引用
出版年
2024
NETL
NSTL
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