In this study we established an HPLC method for the content determination of vitexin and isovitexin in mung bean hull.The Thermo C18 column(4.6 mm×250 mm,5 μm)was adopted,the mobile phase was methanol-0.5%phosphoric acid(40∶60,V/V),the flow rate was 1.0 mL/min,the detection wavelength was 340 nm,and the injection volume was 10 μL.Vitexin and isovitexin had good linear relationship in the concentration ranges of 10.0-80.0 μg/mL and 2.00-30.0 μg/mL(r=0.9999),the average spiked recoveries were 98.89%and 99.39%,and the RSD were 0.21%and 0.26%.The established HPLC method is simple,accurate,sensitive and specific,and can be used for the content determination of vitexin and isovitexin in mung bean hull.
mung bean hullvitexinisovitexincontent determinationHPLC
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