Determination of two constituents in mung bean hull by HPLC
In this study we established an HPLC method for the content determination of vitexin and isovitexin in mung bean hull.The Thermo C18 column(4.6 mm×250 mm,5 μm)was adopted,the mobile phase was methanol-0.5%phosphoric acid(40∶60,V/V),the flow rate was 1.0 mL/min,the detection wavelength was 340 nm,and the injection volume was 10 μL.Vitexin and isovitexin had good linear relationship in the concentration ranges of 10.0-80.0 μg/mL and 2.00-30.0 μg/mL(r=0.9999),the average spiked recoveries were 98.89%and 99.39%,and the RSD were 0.21%and 0.26%.The established HPLC method is simple,accurate,sensitive and specific,and can be used for the content determination of vitexin and isovitexin in mung bean hull.
mung bean hullvitexinisovitexincontent determinationHPLC
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