Comparative study on genomic DNA extraction methods of Salvia miltiorrhiza
In this study we investigated the best method for obtaining genomic DNA from Salvia miltiorrhiza.Ge-nomic DNA was extracted from Salvia miltiorrhiza leaves by different methods.The integrity was evaluated via gel e-lectrophoresis,the concentration and purity were assessed with a micro-spectrophotometer,and the amplification of β-actin was compared.The results showed that the alkali pyrolysis boiling method and the urea method could not provide full length DNA;RNA was present in the DNA extracted by the 4×CTAB method;proteins and phenol were present in the DNA acquired by the SDS ice bath method,the PVP-40 method and the urea method;the DNA ob-tained by the modified urea method had poor amplification effect;β-actin could be amplified with the DNA extrac-ted by the 1.5×CTAB method,the 2×CTAB method and the high salt-low pH method.In conclusion,the DNA ex-tracted by the 2×CTAB method had high concentration and purity,and was suitable for use in subsequent molecular biology studies.
Salvia miltiorrhizagenomic DNA extractionmethod comparisonCTAB
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