Material basis and action mechanism of Ganlexin Capsules in the treatment of Acute Hepatitis
Based on Network Pharmacology and molecular docking technology,we investigated the material basis and action mechanism of Ganlexin Capsules in the treatment of Acute Hepatitis.The chemical components and tar-gets of Ganlexin Capsules were selected in ETCM,Uniprot,HERB,SwissTargetPrediction and TCMSP databases.The disease targets of Acute Hepatitis were selected in DisGeNET,GeneCards and OMIM online databases and in-tersected with the targets of Ganlexin Capsules by Venn diagram.The drug-component-target map was constructed with Cytoscape software.PPI analysis of the intersecting targets was performed by STRING,and the final results of PPI analysis were visualized by Cytoscape.R language was used for GO and KEGG pathway enrichment analysis.GSE27850 and GSE111449 were used for differential gene expression and ROC validation.Finally,molecular doc-king was performed to clarify the main active compounds in Ganlexin Capsules.A total of 236 intersecting genes were obtained,including IL6,PCOLCE,CA3,SERPINE1,EGFR,SELE,etc.The results of GO and KEGG pathway enrichment analysis showed that they were mainly involved in the regulation of PI3K-Akt,TNF and other signaling pathways.Molecular docking showed that the EGFR protein could bind to a variety of components such as vitamin E,lignocaine and paeoniflorin in Ganlexin Capsules.In conclusion,Ganlexin capsule mainly treats Acute Hepatitis by targeting EGFR involved in PI3K-Akt and TNF signaling pathways through lignocaine and paeoniflorin.
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