贵州科学2024,Vol.42Issue(4) :26-32.

追风伞薄层鉴别及不同批次、部位化学成分差异分析

TLC identification of Lysimachia paridiformis var.stenophylla and analysis of its chemical composition

黄桦 杨菁 张敬杰 孙宜春 李慧馨 杨红 邹娟 何康
贵州科学2024,Vol.42Issue(4) :26-32.

追风伞薄层鉴别及不同批次、部位化学成分差异分析

TLC identification of Lysimachia paridiformis var.stenophylla and analysis of its chemical composition

黄桦 1杨菁 1张敬杰 1孙宜春 2李慧馨 2杨红 1邹娟 1何康1
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作者信息

  • 1. 贵州中医药大学 药学院,贵州 贵阳 550025
  • 2. 国药集团同济堂(贵州)制药有限公司,贵州 贵阳 550009
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摘要

目的:建立追风伞薄层色谱(TLC)鉴别方法,分析追风伞不同批次、部位成分差异,为追风伞质量鉴定和资源利用最大化提供依据.方法:以槲皮素和山奈酚为对照品,参照2020年版《中国药典》(四部)通则0502试验,建立追风伞TLC定性鉴别方法,并对21批黔产追风伞不同部位化学成分进行差异分析.结果:21批追风伞不同部位中均具有与对照品位置相同而荧光强度不同的斑点,且斑点圆整、清晰、无干扰,其荧光强度主要表现为叶>全草>茎>根.结论:该方法操作简单,具有较好重复性、耐用性、广泛性和准确性,可初步对黔产追风伞药材进行质量鉴别;追风伞不同批次、部位中所含化合物的种类和含量可能具有一定差异,可进一步探究其活性差异;本实验结果可为后期追风伞质量控制评价及其资源合理利用研究提供数据支撑.

Abstract

In this study we established a thin-layer chromatography(TLC)identification method for Lysimachia pariformis var.stenophylla Franch.,and analyzed the differences in components of Lysimachia pariformis var.steno-phylla of different batches and parts,so as to provide a basis for its quality identification and resource utilization.U-sing quercetin and kaempferol as reference substances,the TLC qualitative identification method was established ac-cording to the general rule 0502 in the Chinese Pharmacopoeia(Volume Ⅳ),and the chemical composition of dif-ferent parts of 21 batches of samples from of Guizhou were analyzed.The 21 batches of L.pariformis var.stenophylla had spots in different parts with the same position as the reference substances and different fluorescence intensity,and the spots were round,clear and without interference.The fluorescence intensity was mainly expressed as leaf>whole grass>stem>root.The method is simple,reproducible,durable,extensive and accurate,and can be used for the quality identification of L.pariformis var.stenophylla.The types and contents of compounds contained in different batches and parts of L.pariformis var.stenophylla may be different,and their activity differences can be further ex-plored.The results of this experiment can provide data support for the quality control of L.pariformis var.stenophylla and the rational utilization of its resources.

关键词

追风伞/薄层鉴别/不同批次/不同部位/化学成分

Key words

Lysimachia paridiformis var.stenophylla Franch./thin-layer identification/different batches/different parts/chemical composition

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基金项目

国药集团同济堂(贵州)技术服务项目(JS-YF-KY-201912015)

出版年

2024
贵州科学
贵州科学院

贵州科学

影响因子:0.395
ISSN:1003-6563
参考文献量11
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