Methodological study on the determination of N-dimethyl nitrosamine in fish products by QuEChERS-EMR-GC/MS
In this study we established a method for the rapid analysis of N-dimethyl nitrosamine in fish products by QuEChERS-EMR-GC/MS.The internal standard was added to the sample,which was extracted with acetoni-trile,purified with QuEChERS-EMR,separated by VF-WaxMS column(60 m × 0.25 mm × 0.25 µm),scanned under single ion detection(SIM)mode,and quantified by internal standard method.N-dimethyl nitrosamine had good linear relationship within the concentration range of 2.0 ng/mL to 50.0 ng/mL,and the correlation coefficient was 0.9994.The limit of detection was 1.0 μg/kg,and the limit of quantitation was 3.0 μg/kg.The recoveries were between 83.1％and 104.3％when the spiked levels were 4.0 µg/kg,20 µg/kg and 40 μg/kg.The relative stand-ard deviation was between 3.3％and 6.1％.The matrix effect of fish substrate on N-dimethyl nitrosamine was 7.5％,showing a weak matrix effect and could be ignored.The method is simple,efficient and environmental protec-tive,with high accuracy and good repeatability,which is suitable for the determination of N-dimethyl nitrosamine in fish products.
万方数据
维普
