低温胁迫对鱼腥草基因表达的影响
Effect of low temperature stress on gene expression of Houttuynia cordata Thunb.
杨振涛 1陈宏宇 1于莹 2邓坤 1杨莉莉 1郑维军 1文爰惠1
作者信息
- 1. 贵州中医药大学 药学院,贵州 贵阳 550025
- 2. 贵州中医药大学 基础医学院,贵州 贵阳 550025
- 折叠
摘要
目的:为研究鱼腥草在低温胁迫下的相关基因表达差异.方法:利用Illumina高通量RNA测序系统对低温胁迫下的鱼腥草叶片样本进行转录组测序.对4h和 15h低温胁迫转录组以及CBF调控因子进行比较探索其是否存在差异.结果:从3 个组织共获得151823 个单基因(unigene),其中75643 个单基因在Nr、COG等7 个数据库中获得注释.与对照相比,4h低温胁迫识别了上调表达基因1715 个,下调表达基因1197 个;而15h低温胁迫识别了上调表达基因4207 个,下调表达基因2600 个.结论:基因功能分析表明,鱼腥草在低温胁迫下可能与细胞缺氧应答、缺水应答和低温应答等过程有关.本实验为鱼腥草低温应答分子机制的相关研究提供参考.
Abstract
To understand the difference in gene expression of Houttuynia cordata Thunb.under low temperature stress,the transcriptomes of H.cordata leaf tissues under low temperature stress were studied by using Illumina high-throughput RNA sequencing.The transcriptomes and CBF regulons under 4 h/15 h low temperature stress was compared.A total of 151823 unigenes were obtained,among which 75643 unigenes were annotated by seven data-bases.There were 1715 up-regulated and 1197 down-regulated unigenes under 4 h low temperature stress,and 4207 up-regulated and 2600 down-regulated unigenes under 15 h low temperature stress.The results of gene func-tion analysis showed that low temperature stress was correlated with cellular response to hypoxia,cellular response to water deprivation,and cellular response to cold.This study has provided reference for research on the cellular mech-anism of low temperature response of H.cordata Thunb.
关键词
鱼腥草/转录组/低温胁迫/基因Key words
Houttuynia cordata Thunb./transcriptome/low temperature stress/gene引用本文复制引用
基金项目
大学生创新创业训练项目(贵中医大创合字[2022]100号)
大学生创新创业训练项目(2015-41300014-100)
贵州中医药大学博士启动基金(贵中医博士启动[2020]19号)
贵州中医药大学博士启动基金(3043-043200019)
出版年
2024
NETL
NSTL
万方数据