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低温胁迫对鱼腥草基因表达的影响

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目的:为研究鱼腥草在低温胁迫下的相关基因表达差异.方法:利用Illumina高通量RNA测序系统对低温胁迫下的鱼腥草叶片样本进行转录组测序.对4h和 15h低温胁迫转录组以及CBF调控因子进行比较探索其是否存在差异.结果:从3 个组织共获得151823 个单基因(unigene),其中75643 个单基因在Nr、COG等7 个数据库中获得注释.与对照相比,4h低温胁迫识别了上调表达基因1715 个,下调表达基因1197 个;而15h低温胁迫识别了上调表达基因4207 个,下调表达基因2600 个.结论:基因功能分析表明,鱼腥草在低温胁迫下可能与细胞缺氧应答、缺水应答和低温应答等过程有关.本实验为鱼腥草低温应答分子机制的相关研究提供参考.
Effect of low temperature stress on gene expression of Houttuynia cordata Thunb.
To understand the difference in gene expression of Houttuynia cordata Thunb.under low temperature stress,the transcriptomes of H.cordata leaf tissues under low temperature stress were studied by using Illumina high-throughput RNA sequencing.The transcriptomes and CBF regulons under 4 h/15 h low temperature stress was compared.A total of 151823 unigenes were obtained,among which 75643 unigenes were annotated by seven data-bases.There were 1715 up-regulated and 1197 down-regulated unigenes under 4 h low temperature stress,and 4207 up-regulated and 2600 down-regulated unigenes under 15 h low temperature stress.The results of gene func-tion analysis showed that low temperature stress was correlated with cellular response to hypoxia,cellular response to water deprivation,and cellular response to cold.This study has provided reference for research on the cellular mech-anism of low temperature response of H.cordata Thunb.

Houttuynia cordata Thunb.transcriptomelow temperature stressgene

杨振涛、陈宏宇、于莹、邓坤、杨莉莉、郑维军、文爰惠

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贵州中医药大学 药学院,贵州 贵阳 550025

贵州中医药大学 基础医学院,贵州 贵阳 550025

鱼腥草 转录组 低温胁迫 基因

大学生创新创业训练项目大学生创新创业训练项目贵州中医药大学博士启动基金贵州中医药大学博士启动基金

贵中医大创合字[2022]100号2015-41300014-100贵中医博士启动[2020]19号3043-043200019

2024

贵州科学
贵州科学院

贵州科学

影响因子:0.395
ISSN:1003-6563
年,卷(期):2024.42(5)