Optimization of content determination method for Cactus Weikang Capsules
We optimized the content determination method for Cactus Weikang Capsules,so as to improve the convenience and scientificity of the method.The sample was extracted by heating reflux,and the content of hesperi-din in the sample was determined by HPLC.Chromatographic conditions;the chromatographic column was Agilent TC-C18 column(250 mm×4.6 mm,5 μm),the mobile phase was acetonitrile-0.1%phosphoric acid solution(20∶80),the column temperature was room temperature,the PDA detector was adopted,the detection wavelength was 283 nm,and the flow rate was 1.0 mL/min.The results showed that hesperidin had good linear relationship in the range of 2.0742 μg/mL to 414.8496 μg/mL(R2=0.9997);the precision RSD≤0.56%,the repeatability RSD≤1.12%(n=6),and the stability RSD≤2.03%;the average recovery rate of the sample was 94.60%,RSD=2.31%(n=6)when heated for 30 minutes with 25 mL methanol as the extraction solvent.The optimized HPLC method is simple,accurate and reproducible,and can be used for the determination of hesperidin in Cactus Weikang Capsules.This study has provided scientific basis for the quality control of Cactus Weikang Capsules.
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