Molecular identification of three varieties of tea chrysanthemum in the market
In order to accurately identify the tea chrysanthemum varieties in the market and further standardize the management and supervision of the tea chrysanthemum market,four common DNA barcode fragments(ITS,rbcL,psbA-trnH and matK)were selected to conduct molecular identification study on three tea chrysanthemum varieties from different origins.A total of 117 sequences were obtained from the three tea chrysanthemum varieties.The ITS fragment had the best discrimination.All 24 samples of Jinsi chrysanthemum clustered together in the NJ tree con-structed with ITS.In conclusion,compared with rbcL and psbA-trnH,ITS fragment has better resolution of tea chrys-anthemum varieties.However,in order to thoroughly clarify the phylogenetic relationship between various tea chrys-anthemum varieties,molecular systematic study at the genomic level is needed.
Jinsi chrysanthemumWuyuan chrysanthemumHangbai chrysanthemumDNA barcodeITSrbcLps-bA-trnH
NETL
NSTL
万方数据
