以1,2-萘醌-4-磺酸钠为显色剂测量水溶液中哌嗪含量的快速测量方法研究
Rapid detection of piperazine in aqueous solution with sodium 1,2-naphthoqui-none-4-sulfonate as the chromogenic reagent
陆永霞1
作者信息
- 1. 安徽省计量科学研究院,安徽 合肥 230051
- 折叠
摘要
试验建立了一种基于分光光度法测量哌嗪在水溶液中含量的检测新方法.采用 1,2-萘醌-4-磺酸钠(NQS)为显色剂、在pH为11 的 Na2 HPO4(0.05 mol/L)-NaOH(0.1 mol/L)缓冲液中,哌嗪与NQS反应生成砖红色产物,其在波长为486 nm处有最大吸收峰.哌嗪水溶液浓度在 10～30 μg/mL的范围内显现出良好的线性关系.线性回归方程为y=0.0702x-0.362,线性相关系数r=0.9997,其表观摩尔吸光系数为1.8×104 L·(mol·cm)-1,检出限(3 s/k)为 0.81 μg/mL,测量精密度为0.71%(n=11),相对标准偏差RSD=1.37%(n=11).本方法可以直接测量生产过程水溶液中哌嗪的含量,通过加标回收率试验测得回收率在94.0%～105.5%之间.
Abstract
A new method for the determination of piperazine in aqueous solution was established based on spectro-photometry.Piperazine reacted with sodium 1,2-naphthoquinone-4-sulfonate(NQS)in Na2 HPO4(0.05 mol/L)-NaOH(0.1 mol/L)buffer at pH 11 to form a brick red product,which had a maximum absorption peak at 486 nm.The linear relationship was good in the concentration range of 10 μg/mL to 30 μg/mL.The linear regression e-quation was y=0.0702x-0.362,and the linear correlation coefficient r=0.9997.The apparent molar absorptivity was 1.8×104 L·(mol·cm)-1.The detection limit(3 s/k)was 0.81 μg/mL,the precision was 0.71%(n=11),and the relative standard deviation was 1.37%(n=11).The method could directly measure the content of pipera-zine in aqueous solution in the production process,and the spiked recovery rate was between 94.0%and 105.5%.
关键词
哌嗪/分光光度法/1/2-萘醌-4-磺酸钠(NQS)/回收率Key words
piperazine/spectrophotometry/sodium 1/2-naphthoquinone-4-sulfonate(NQS)/recovery rate引用本文复制引用
出版年
2024
NETL
NSTL
万方数据