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花榈木种子休眠和萌发的初步研究

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为探讨花榈木种子休眠的原因和萌发特性,对花榈木种子的物理性状、吸水性、发芽条件进行了研究,并对其种皮和种胚的甲醇浸提液进行了生物测定.结果表明:花榈木种子的种皮坚硬致密,透水透气性较差,用浓硫酸酸蚀处理2 h能有效改善花榈木种子的种皮透性,其72 h累积吸水率达96.3%,比对照增加93%.花榈木种子萌发受到种皮限制,以全部去除种皮,恒温25℃发芽效果最好,发芽率达80%.花榈木种子的种皮和种胚中存在抑制白菜种子萌发的物质.随着种皮、种胚浸提液浓度的增加,白菜种子发芽率逐渐降低.其中,种皮浸提液的抑制作用最大,0.01 g/mL、0.05 g/mL和0.1 g/mL种皮甲醇浸提液处理的白菜种子发芽率分别比对照降低13%、27%和39%.初步认为,导致花榈木种子休眠的原因是种皮透性差,种皮和种胚中含有抑制物质.
A Preliminary Study on Dormancy and Germination of Ormosica ihernryi Seeds
To explore the dormant reasons and germination character of Ormosia henryi seeds, the physical character, water-absorbing character and germination condition were studied, and the methanol extract from Ormosia henryi seed coat and endosperm were measured by bioassay. The results showed that Ormosia henryi seed coat is hard and compact, less permeable breathable, with concentrated sulfuric acid etching for 2 hours can improve the seed coat permeability. The 72 hour cumulative water-absorbing percentage is 96.3%, compared with the control increases of 93%. The seed germination of Ormosia henryi is restricted by seed coat, removing all coat and germinating under 25℃ are the best, with 80%germination rate. The inhibitor substance contained in the seed coat and embryo of Orrnosia henryi can restrain Brassicca carnpestris seed germinating, the germination rate of Brassicca campestris seed decreased as the extract solution concentration increased. The seed coat extract has the maximum inhibition, the germination rate of Brassica campestris seeds treated with 0.01 g/mL, 0.05 g/mL and 0.1g/mL seed coat methanol extract reduced 13% , 27% and 39% respectively compared with the control. It is preliminarily thought that one of the reasons causing the seed dormancy of Ormosia henryi is poor seed coat permeability, the other one is inhibitor substance contained in seed coat and endosperm.

Ormosia henryiseed dormancygerminationbioassayinhibitor substance

邓兆、韦小丽、孟宪帅、冯明桥、成开国、潘昌贵

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贵州大学林学院,贵州贵阳550025

花榈木 种子休眠 萌发 生物测定 抑制物质

贵州省省长基金国家大学生创新计划项目贵州省林业厅项目

黔省专合字200901]109国创009黔林科合2010重大02

2011

贵州农业科学
贵州省农业科学院

贵州农业科学

CSTPCD北大核心
影响因子:0.642
ISSN:1001-3601
年,卷(期):2011.39(5)
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