With the purpose of getting the optimum fermentation condition of alkalescence cellulase produced by B. Pumilus SI 2, the fermentation condition was optimized through the single factor and orthogonal design. The results showed that the bran and CMC-Na were the suitable carbon source and the best nitrogen source was yeast. The enzyme producing rate of organic nitrogen sources was higher than that of inorganic nitrogen sources. The optimal conditions were as follows: initial pH 8.5, culture temperature 32℃,70 mL media filled in the 250 mL triangle flask, the inoculation amount 3%(v/v), fermentation time 20 h. The alkaline cellulase activity reached 367. 9 g/mL under the optimal condition.
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维普
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