In order to ensure the quality of pitaya fruit juice beverage and filter out the best sterilized method, Prepared pitaya fruit juice beverage was sterilized with different sterilized ways such as water bath,microwave heating,ozone sterilization through the sensory evaluation, determination of mecrobes and physico-chemical parameters, then they were compared using Duncan's honestly significant difference multiple comparison test. The results showed that the best sterilization process was microwave heating for 1. 5 min. The sterilized pitaya fruit juice beverage contained amino nitrogen 4. 85 mg/100 mL and Vc 0. 85 mg/L, and the sterilization process had little destructiveness to the nutritional components such as total sugar, amino nitrogen and Vc.