Screening and Identification of Chitinophaga pinensis ZLX 3 and Chitinophaga rupis sp.MJM 38 Producing Anticoagulation and Thrombolysis Double Active Substance
To screen the Chitinophaga strains that can produce the antithrombotic active substance, the authors screened the target strains on VY/4 plate from dozens of samples such as rabbit manure,sheep manure,soil,deadwood,etc.and detected the fibrinolytic activity of the target strains by the fibrin plate and the anticoagulant activity by fibrin tube, then identified the target strains by morphological characteristics,physical and chemical properties and G+C content of DNA and 16S rRNA sequence homology.Results:the strains ZLX 3 and MJM 38 that can produce antithrombotic active substance were obtained from the rotten wood samples.And found that the extracellular fluid of the strains ZLX 3 and MJM 38 showed both obvious fibrinolytic activity and obvious anticoagulation activity.The strain ZLX 3 belonged to the Chitinophaga pinensis and the strain MJM 38 belonged to the Chitinophaga rupis.The strains ZLX 3 was named as Chitinophaga pinensis ZLX 3 and the strains MJM 38 as Chitinophaga rupis sp.MJM 38. Conclusion:Both the strains ZLX 3 and the strains MJM 38 can produce obvious anticoagulation and thrombolysis double active substance.
NETL
NSTL
万方数据
维普
