基于 SSR 标记的玉米自交系遗传多样性
Genetic Diversity of Maize lnbred Lines in Chongqing
邸仕忠 1刘红芳 2孙小红 1秦密 2罗永统 1陈维竟 1杨飞2
作者信息
- 1. 重庆市渝东南农业科学院，重庆 涪陵 408000
- 2. 长江师范学院 生命科学与技术学院，重庆 涪陵 408100
- 折叠
摘要
为了解重庆市玉米自交系的遗传多样性，提高育种效率，利用32对 SSR引物对66份玉米自交系进行检测，分析其遗传相似系数，并进行聚类分析，掌握材料间的亲缘关系。结果表明：1）共检测出307个条带，每对引物检测到条带4～25个，平均9．59个，其中多态性条带占80．78％；多态性信息量（PIC 值）变幅为0．516～0．988，平均为0．721；供试自交系间遗传相似系数变化范围为0．54～0．88，平均0．68，遗传相似系数在0．60～0．70间分布最多，占60．33％，供试材料亲缘关系较近，但也有一定的遗传差异。2）聚类分析将供试材料分为6大类群，其中，改良 Reid 群和旅大红骨类群分别占24．24％和60．60％。
Abstract
The genetic similar coefficient of 66 maize inbred lines was determined by 32 pairs of SSR primers and their genetic relationship was analyzed by clustering analysis to study the genetic diversity of maize inbred lines and improve breeding efficiency in Chongqing.The results showed that:1)307 bands are detected by 32 pairs of primers and 4-25 bands can be detected by a pairs of primers. The polymorphism band accounts for 80.78% and the variation range of PIC is 0.516~0.988 with an average of 0.721.The variation range of genetic similar coefficient among tested inbred lines is 0.54~0.88 with an average of 0.68.Most genetic similar coefficient is 0.60~0.70,accounts for 60.33%,which indicates that the genetic relationship among tested inbred lines is closer but there still is a certain difference in genetic relationship;2)66 inbred lines can be divided into 6 groups.The modified Reid group and Ludahonggu group accounts for 24.24% and 60.60% respectively. Other germplasm groups, especially tropical germplasm resources should be used in maize breeding in Chongqing.
关键词
玉米/自交系/SSR/标记/遗传多样性/重庆Key words
maize/inbred line/SSR marker/genetic diversity/Chongqing引用本文复制引用
基金项目
重庆市涪陵区科委项目(FLKJ2013BBB2129)
出版年
2014
国家科技期刊平台
NSTL
万方数据