为巨菌草的种质鉴定、遗传育种和亲缘关系提供一定的细胞学基础,采用传统压片法,对象草(Pennisetum purpureum schumach)和巨菌草〔Pennisetum giganteum z.x.lin(暂定名)〕的染色体数目及核型进行分析。结果表明:象草和巨菌草的染色体形态较为一致,大部分由中部(m)和近中部(sm)着丝粒的染色体组成,象草和巨菌草的核型公式分别为2 n=4 x=28=20 m+8 sm 和2 n=4 x=28=16 m+8 sm+4 st;染色体相对长度组成分别为2 n=4 x=28=6 L+6 M2+10 M1+6 S 和2 n=4 x=28=4 L+8 M2+10 M1+6 S,染色体基数和总数目一致,核型分类均为2 A 型,不对称系数为58%~60%,均属于较原始的类型。结论:象草和巨菌草的的染色体无明显差异性,从细胞染色体水平上认为两者亲缘关系较近。
Karyotype Analysis of Pennisetum purpureum and Pennisetum giganteum
To provide certain cellular basis for germplasm, breeding and genetic kinship of P .giganteum,The karyotype analysis and chromosome number of P .purpureum and P .giganteum were conducted by traditional squash method.The results showed that the karyotype morphology of materials were similar,consisted of metacentric chromosomes and sub-metacentric chromosomes mostly.In this study, the karyotype formulas of P . purpureum and P . giganteum were respectively 2 n=4 x=28=20 m+8 sm,2 n=4 x=28= 16 m+8 sm+4 st.Constitutions of relative length of two plants were 2 n=4 x=28=6 L+6 M2 + 10 M1 +6 S,2 n=4 L+8 M2 + 10 M1 + 6S.The karyotype of P .purpureum was the same as that of P .giganteum in chromosome basic number and total number.And two varieties were classed as 2A.The asymmetry coefficient were in the range of 58% to 60%,which demonstrated basic symmetry karyotypes. Conclusion: Both of the two plants had no significant differences in chromosomes between them and the relationship between them was close from cellular level.