Total DNA Extraction from Artemisia argyi and Optimization on ISSR-PCR Reaction System
In order to explore the genetic diversity of A.argyi,an orthogonal experimental design was employed combined with single factor analysis to optimize the five main factors(Mg2+ ,primers,template DNA,dNTPs and Taq DNA enzyme)of PCR reaction in four levels.Results:The optimal ISSR-PCR reaction system:20 μL system,template DNA 60 ng,Mg2+ 2.5 mmol/L,primer 0.8 μmol/L,dNTPs 0.15 mmol/L,Taq DNA enzyme 1.0 U.The results of range analysis showed that the order of the influence degree to ISSR was Mg2+ > primer> template DNA> dNTPs > Taq DNA enzyme.
Artemisia argyimolecular markerISSRtraditional Chinese medicine
NETL
NSTL
万方数据
