Isolation and Identification of Antagonistic Bacteria against Pectobacterium carotovorum in Pinelia ternate
The biocontrol strains with more strong antagonistic effects on P.carotovorum of P.ternate were screened from soil samples of various regions in Guizhou by plate dilution and plate confront culture methods and then the isolated strains were identified by morphological observation, physiological and biochemical detection and 16S rPNA sequence analysis to promote green cultivation of P.ternate in Guizhou.Results: 270 bacteria strains are isolated and a strain, HM9-1 with more strong antagonistic effect, is screened from 270 bacteria stains.HM9-1 strain with 2.4 cm of inhibition zone against P.carotovorum is identified as B.megatherium.