Extraction and Antioxidant Activity of Total Flavonoids from Rubus idaeopsis Focke Leaves
The extraction technology of total flavonoids from Rubus idaeopsis Focke Leaves was optimized by the orthogonal test and the antioxidant activity of flavonoids from Rubus idaeopsis Focke Leaves was evaluated by using the clearance rate of DPPH and ABTS+ · to fully explore and utilize Rubus idaeopsis Focke resources in Guizhou.Results:The optimum extraction conditions for total flavonoids from Rubus idaeopsis Focke Leaves are 70％ ethanol,1 ∶ 60 of material-liquid ratio (g/ml) and two extractions at 80℃ for 4h/extraction.Total flavonoids content can reach 38.540 mg/g under the optimum extraction conditions.Total flavonoids extracted from Rubus idaeopsis Focke Leaves is of the strong clearance rate against DPPH and ABTS+ ·.The clearance ability of total flavonoids from Rubus idaeopsis Focke Leaves during 10.32～19.28 μg/mL against DPPH and ABTS+ · is well above VC and BHT.Rubus idaeopsis Focke Leaves containing abundant total flavonoids with strong antioxidant ability should be utilized as a hatural antioxidant.
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