Optimization of Extraction condition for Total Alkaloids from Dendrobium nobile
In order to scientifically and rationally exploit medicinal resources of D.nobile,a singlefactor experiment was conducted to analyze the effects of extraction temperature,ethanol concentration,extraction time,solid-liquid ratio and extraction times on total alkaloids from D.nobile,and the acid-base titration method was applied in determination of total alkaloids.On the basis of this test,an orthogonal experimental design test was applied to further investigate the extraction process for total alkaloids from D.nobile,the concentration of ethanol aqueous solution,extraction time,solid-liquid ratio and times of extraction were reviewed seriously.Results:The optimum extraction process of total alkaloids from D.nobile included extraction temperature 85℃,90％ ethanol concentration,extraction time 3 h,solid-liquid ratio 1 ∶ 20 g/mL,two times of extraction.The average extraction rate under this optimum condition for total alkaloids from D.nobile was 87.8 mg/g.
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