应用STR荧光标记分析烟台地区菊花种质资源遗传多样性
Genetic Diversity of Chrysanthemum Germplasm Resources in Yantai by Using STR Fluorescence Markers
朱胜波 1李恺睿 1樊铭玺 1焦睿 1陈晓峰1
作者信息
- 1. 中国农业大学烟台研究院,山东烟台 264670
- 折叠
摘要
[目的]探明烟台地区菊花种质资源的遗传多样性,为烟台地区菊花种质的分类鉴别、资源保护及品种培育提供依据.[方法]应用荧光STR分子标记和毛细管电泳检测技术分析烟台地区非洲菊、秋菊、洋甘菊等12份菊花种质,结合主要表型性状观察进行聚类分析并构建分子身份证.[结果]10对引物共检测到103个STR等位基因位点,平均每对引物的位点数10.3个,扩增片段长度在118～376 bp之间,引物多态性信息(PIC)平均0.795 8.聚类分析依照遗传相似系数将12份菊花种质分为四大类,其中,Ⅰ类群包括秋菊、雏菊、野菊(1)、杭菊(白)、杭菊(黄)、翠菊、野菊(2)共7份种质,占总数的58.3％,含大菊1份、小菊6份,平瓣2份、匙瓣3份、管瓣2份;Ⅱ类群包括非洲菊、洋甘菊2份种质,占总数的16.7％,花瓣类型均为平瓣;Ⅲ类群仅杭菊(紫)1份种质,占总数的8.3％;Ⅳ类群包括一年蓬、野菊(3)2份种质,占总数的16.7％,均为小菊、平瓣花.不同花色、花序大小、花瓣类型的种质存在交叉聚集现象.[结论]烟台地区菊花种质具有较好的遗传多样性,STR荧光标记可有效分析该类种质资源,基于STR分子手段构建了 12份菊花种质资源的分子身份证.
Abstract
[Objective]The genetic diversity of chrysanthemum germplasm resources in Yantai area was studied,which supported the classification,resource protection,and variety cultivation of chrysanthemum germplasm in Yantai area.[Method]Fluorescent STR molecular markers and capillary electrophoresis were used to analyze 12 chrysanthemum germplasms from Yantai area,and cluster analysis was conducted based on the observation of major phenotypic traits to construct molecular ID.[Result]A total of 103 STR alleles were detected from 10 pairs of primers,with an average of 10.3 alleles per primer pair.The lengths of the amplified fragment were 118-376 bp,and the primer polymorphism information(PIC)averaged 0.795 8.Cluster analysis of chrysanthemum germplasm was conducted,and 12 germplasm were divided into 4 categories based on genetic similarity coefficient.Group Ⅰ included Chrysanthemum morifolium'Qiuju',Bellis perennis,Chrysanthemum indicum(1),Chrysanthemum mori folium'Hangju'(white),Chrysanthemum morifolium'Hangju'(yellow),Callistephus chinensis and Chrysanthemum indicum(2),which accounted for 58.3％of 12 chrysanthemum germplasms,including 1 large-flowered chrysanthemum,6 small-flowered chrysanthemums,2 flat-type petals,3 spoon-type petals,2 tubular type petals.Group Ⅱ included Gerbera jamesonii and Matricaria recutita,which accounted for 16.7％of total chrysanthemums,and their petals were all flat type.Only Chrysanthemum morifolium'Hangju'(purple)belonged to Group Ⅲ,which accounted for 8.3％of total chrysanthemums.Group Ⅳ included Erigeron annuus and Chrysanthemum indicum(3),accounting for 16.7％,and the two cultivars were both small-flowered chrysanthemums with flat-type petals.There was cross and aggregation in different flower color,inflorescence size and petal type.[Conclusion]Chrysanthemum germplasm in Yantai area has good genetic diversity,and STR fluorescence markers can effectively analyze the type of germplasm resources.The molecular ID of 12 chrysanthemum germplams were constructed based on STR molecular method.
关键词
STR荧光标记/菊花/遗传多样性/种质资源/分子身份证Key words
STR fluorescent marker/chrysanthemum/genetic diversity/germplasm resource/molecu-lar ID引用本文复制引用
基金项目
国家重点研发计划(2018YFD1000800)
中国农业大学本科生URP项目(U2022066)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据