基于ITS2对家蚕人工饲料霉变病原微生物的检测
Detection of Pathogenic Microorganism of Mildew in Artificial Diet for Bombyx mori Based on ITS2
邹邦兴 1卿圣环 1张宇靖 1蒋亚明 1张友洪 1肖文福 1周安莲1
作者信息
- 1. 四川省农业科学院蚕业研究所,四川南充 637000
- 折叠
摘要
[目的]探明引起家蚕人工饲料霉变的病原微生物,为制定防止家蚕人工饲料霉变决策提供科学依据.[方法]对饲料霉变区域随机取样,对其ITS2区域进行序列扩增检测,通过OTU聚类、物种注释和Alpha多样性分析其在不同分类水平下的物种组成.[结果]引起家蚕人工饲料霉变致病微生物主要生物学分类为真菌界(Fungi)、子囊菌门(Ascomycota,99.98％～100％)、散囊菌纲(Eurotiomycetes,99.80％～99.97％)、散囊菌 目(Eurotiomycetes,99.80％～99.97％)、毛刷囊菌科(T richocomaceae,99.80％～99.97％)、石座菌属(Petromyces,99.80％～99.96％)、黄曲霉菌(Aspergillus flavus,99.75％～99.91％).[结论]引起家蚕人工饲料霉变的病原菌主要为真菌界的黄曲霉菌.
Abstract
[Objective]The pathogenic microorganisms of mildew in artificial diet for Bombyx mori were studied,which provided scientific reference for preventing the mildew of artificial feed for B.mori.[Method]Feed mildew regions were randomly sampled for sequence amplification in the ITS 2 region,and their species composition at different taxonomic levels were analyzed by OTU clustering,species annotation and Alpha diversity.[Result]The main biological classification of pathogenic microorganisms causing moldy artificial feed was fungi,Ascomycota(99.98％-100％),Eurotiomycetes(99.80％-99.97％),Eurotiomycetes(99.80％-99.97％),Trichocomaceae(99.80％-99.97％),Petromyces(99.80％-99.96％),Aspergillus flavus(99.75％-99.91％).[Conclusion]The main pathogenic bacteria that cause feed mildew is Asperillus flavus.
关键词
家蚕/人工饲料/霉变/微生物/ITS2区域Key words
Bombyx mori/artificial feed/mildew/microorganism/ITS 2 region引用本文复制引用
基金项目
现代农业产业技术体系建设项目(CARS-18)
四川省农科院自主创新专项(2022zzcx084)
南充市研发项目(21YFZJ0091)
出版年
2024
国家科技期刊平台
NSTL
万方数据