Cloning and Expression Analysis of CqANS Gene from Chenopodium quinoa
[Objective]Through bioinformatics analysis of anthocyanidin synthase(ANS)gene in Chenopodium quinoa,its relative protein traits were studied,which provided basis for further studying the molecular mechanism of role of CqANS gene.[Method]The cDNA full-length sequence of C.quinoa ANS gene(CqANS)was cloned by PCR reaction,and then the sequence and characteristics of protein encoded by CqANS were predicted and analyzed.And real-time quantitative PCR was used to analyze the expression of CqANS under different stress treatments.[Result]The CqANS contained two exons and one intron,and the protein encoded by the CqANS encoded 359 amino acid residues.The CqANS was a hydrophilic protein,which had no signal peptide and was located in the cytoplasm.CqANS belonged to the PLN03178 superfamily and had a typical conservative domain of the Fe2+-dependent dioxygenase family(2OG-FeⅡ_Oxy).Promoter analysis showed that there were many cis-acting elements in the promoter region of CqANS in response to abiotic stress.Low temperature stress could induce the expression of CqANS,while exogenous ABA treatment and drought treatment could inhibit the expression of CqANS.CqANS interacted with several proteins,including AUR62014624-RA.[Conclusion]The ANS gene of C.quinoa(CqANS)has the characteristics of ANS gene family and may has similar biological functions as ANS genes in other plants.
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