首页|魔芋茎腐病病原的生物学特性及不同杀菌剂对病原菌的室内毒力

魔芋茎腐病病原的生物学特性及不同杀菌剂对病原菌的室内毒力

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[目的]探明魔芋茎腐病病原的生物学特性及不同杀菌剂对病原的室内毒力,为精准防控魔芋茎腐病提供理论依据.[方法]以魔芋茎腐病病原Fusarium solani为研究对象,采用菌丝生长速率法研究碳源、氮源、培养基及pH对其菌丝生长的影响,并通过室内毒力测定F.solani对 75%肟菌·戊唑醇(WG)和 400 g/L氟硅唑(EC)等 14种杀菌剂的敏感性.[结果]F.solani对果糖和硝酸钾利用率较高,菌丝生长速率分别为 0.931 cm/d和 0.914 cm/d;适合用OA或PDA作为基础培养基,菌丝生长速率分别为 0.879 cm/d和 0.817 cm/d.F.solani最适pH为 8,其菌丝生长速率为 0.821 cm/d.病原菌丝在≥65℃温度下处理 10 min后无生长活性.75%肟菌·戊唑醇(WG)和 400 g/L氟硅唑(EC)对 F.solani毒力较高,EC50 分别为 0.890 0 μg/mL和1.002 5 μg/mL.[结论]F.solani利用率最高的碳源是果糖,氮源是硝酸钾,适合在OA或PDA中培养及弱碱性环境中生存,菌丝致死温度为65℃.肟菌·戊唑醇和氟硅唑对魔芋茎腐病防效较高.
Biological Characteristics of Konjac Stem Rot Pathogen and Its Laboratory Toxicity Effected by Different Fungicides
[Objective]The biological characteristics of the pathogens of konjac stem rot and its laboratory toxicity influenced by different fungicides were investigated,which provided theoretical basis for accurate prevention and control of konjac stem rot.[Method]Fusarium solani,a pathogen of konjac stem rot,was taken as the research object.The effects of carbon source,nitrogen source,medium and pH on the mycelial growth were studied by mycelial growth rate method,and the sensitivities of F.solani to 14 kinds of fungicides were determined by laboratory toxicity test.[Result]F.solani had a high utilization rate of fructose and potassium nitrate,and the growth rate of mycelium was 0.931 cm/d and 0.914 cm/d,respectively.OA or PDA were suitable as the basal medium,and the growth rate of mycelia was 0.879 cm/d and 0.817 cm/d,respectively.F.solani had an optimum pH of 8 and a mycelial growth rate of 0.821 cm/d.The pathogen mycelia showed no growth activity treated with temperatures≥65℃ for 10 min.The EC50 of 75％trifloxystrobin·tebuconazole(WG)and 400 g/L flusilazole(EC)against F.solani were 0.890 0 μg/mL and 1.002 5 μg/mL,respectively.[Conclusion]The most utilized carbon source of F.solani is fructose,and the nitrogen source is potassium nitrate,which are both suitable for cultivation in OA or PDA and survival in weakly alkaline environment.The lethal temperature to mycelium is 65℃.Trifloxystrobin·tebuconazole and flusilazole have high-efficient prevention and control of konjac stem rot.

konjacstem rot of konjacFusarium.solanibiological characteristicsfungicidemycelial growth ratelaboratory toxicity

刘思睿、赵兴丽、罗林丽、周玉锋

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贵州省生物技术研究所,贵州贵阳 550006

贵州省农业生物技术重点实验室,贵州贵阳 550006

贵州省茶叶研究所,贵州贵阳 550006

魔芋 魔芋茎腐病 腐皮镰刀菌 生物学特性 杀菌剂 菌丝生长速率 室内毒力

贵州省科技支撑计划项目贵州省科技计划项目&&贵州省农业科学院青年科技基金

黔科合支撑[2021]一般208黔科合服企[2019]4009黔科合服企[2021]11黔农科院青年科技基金[2021]18

2024

10.3969/j.issn.1001-3601.2024.02.008

贵州农业科学
贵州省农业科学院

贵州农业科学

CSTPCD
影响因子：0.642
ISSN：1001-3601
年,卷(期)：2024.52(2)
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