Genes Responsive to Mechanical Demage in Pinellia ternata Leaves Based on Transcriptome Analysis
[Objective]The differences in expressed genes responsive to mechanical demage in Pinellia ternata leaves were explored to provide a reference for further research on responsive mechanism to stress of P.ternata.[Method]The transcriptome sequence was performed on P.ternata leaves under the stress of mechanical demage,and the gene expression and regulatory pathway were compared and analyzed.[Result]A total of 246 711 unigenes were obtained from 3 tissues,among which 117 798 unigenes were annotated in four databases such as Nr and COG.There were 7 895 and 22 972 responsive genes in cut-mechanical demage and pierce-mechanical demage in P.ternata.Among the responsive genes,several gene members of mechanical demage stress were found.[Conclusion]The mechanical demage responsive process of P.ternata may be correlated with several processes such as defense response,response to demage and chloroplast thylakoid membrane in P.ternata.
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