基于转录组分析鉴定半夏叶片机械损伤应答基因
Genes Responsive to Mechanical Demage in Pinellia ternata Leaves Based on Transcriptome Analysis
陈宏宇 1李伟忠 1梁春波 1梁彦涛 1檀龙颜 1于莹2
作者信息
- 1. 贵州中医药大学药学院,贵州贵阳 550025
- 2. 贵州中医药大学基础医学院,贵州贵阳 550025
- 折叠
摘要
[目的]探明半夏叶片在机械损伤胁迫应答方面的基因表达差异,为进一步研究半夏胁迫应答机制提供参考.[方法]对半夏在机械损伤胁迫下的叶片进行转录组测序,并比较分析基因表达与调控通路.[结果]从 3个组织共获得 246 711个单基因(Unigene),其中 117 798个基因在Nr、COG等 4个数据库中获得注释.剪切型机械损伤和针刺型机械损伤分别识别 7 895个和 22 972个应答基因.在应答基因中,发现多个机械损伤胁迫的基因成员.[结论]半夏机械损伤应答过程可能与防御应答、损伤应答和叶绿体类囊体膜等多个过程有关.
Abstract
[Objective]The differences in expressed genes responsive to mechanical demage in Pinellia ternata leaves were explored to provide a reference for further research on responsive mechanism to stress of P.ternata.[Method]The transcriptome sequence was performed on P.ternata leaves under the stress of mechanical demage,and the gene expression and regulatory pathway were compared and analyzed.[Result]A total of 246 711 unigenes were obtained from 3 tissues,among which 117 798 unigenes were annotated in four databases such as Nr and COG.There were 7 895 and 22 972 responsive genes in cut-mechanical demage and pierce-mechanical demage in P.ternata.Among the responsive genes,several gene members of mechanical demage stress were found.[Conclusion]The mechanical demage responsive process of P.ternata may be correlated with several processes such as defense response,response to demage and chloroplast thylakoid membrane in P.ternata.
关键词
半夏/转录组/机械损伤/基因Key words
Pinellia ternata/transcriptome/mechanical demage/gene引用本文复制引用
基金项目
贵州省科技厅基础研究计划项目(黔科合基础-ZK[2022]一般469)
贵州中医药大学博士启动基金(贵中医博士启动[2020]19号)
贵州中医药大学博士启动基金(3043-043200019)
出版年
2024
国家科技期刊平台
NSTL
万方数据