Effects of Gender and Habitat Factors on Intestinal Flora Diversity of Apodemus peninsulae
[Objective]The effects of different factors on the structure and diversity of intestinal flora in Apodemus peninsulae were explored to lay the theoretical basis for the further study of environmental adaptability mechanism of A.peninsulae.[Method]The 16S rRNA V3-V4 regions of cecum samples in A.peninsulae were sequenced by Illumina high throughput sequencing technology to analyze the effects of gender,habitat and other factors on intestinal flora structure and diversity of A.peninsulae.[Result]At the phylum level,the dominant bacteria of intestinal flora in A.peninsulae were Firmicutes(female 66.266%,male 61.866%)and Bacteroidetes(female 15.478%,male 12.019%).At the genus level,the dominant bacteria were unclassified Lachnospiraceae(female 24.665%,male 24.714%),Lachnospiraceae NK4A136 Group(female 19.313%,male 11.612%)and unclassified Muribaculaceae(female12.962%,male 9.040%).The intestinal flora structure of female A.peninsulae samples showed high similarity,while the male A.peninsulae samples showed great difference.The intestinal flora structure of A.peninsulae samples in broad-leaved forest was highly similar,while the intestinal flora structure of A.peninsulae samples in coniferous and broad-leaved mixed forest was greatly different.[Conclusion]Different gender and habitat factors had great effects on intestinal flora structure of A.peninsulae.
NETL
NSTL
万方数据
